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A major vitamin D metabolite was isolated in pure form from the blood plasma of chicks either maintenance levels or large doses of vitamin D3. The isolation involved methanol-chloroform extraction and five column chromatographic procedures. The metabolite purification and elution position on these columns were followed by a competitive protein binding assay. The metabolite was identified, using high- and low-resolution mass spectrometry, 270-MHz proton nuclear magnetic resonance spectrometry, ultraviolet absorption spectrophotometry, Fourier transform infrared spectrophotometry, and specific chemical reactions, as 3 beta,-25-dihydroxy-9,10-seco-5,7,10(19)-cholestatrieno-26,23-lactone. The trivial names 25-hydroxyvitamin D3 26,23-lactone or calcidiol 26,23-lactone are suggested for this compound.
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
October 1980,
Archives of biochemistry and biophysics,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
August 1982,
The Journal of biological chemistry,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
February 1982,
FEBS letters,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
November 1981,
FEBS letters,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
December 1982,
The Journal of biological chemistry,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
January 1984,
The Journal of biological chemistry,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
March 1982,
FEBS letters,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
August 1981,
Proceedings of the National Academy of Sciences of the United States of America,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
December 1981,
Archives of biochemistry and biophysics,
J K Wichmann, and
H F DeLuca, and
H K Schnoes, and
R L Horst, and
R M Shepard, and
N A Jorgensen
February 1995,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
