BIOMAT Database Logo BIOMAT Database Logo

[A bioluminescent solid phase for immunoassays and the detection of nucleic probes]. 1990

J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
INSERM Unité 58, Montpellier.

A luminescent adsorbent constituted of bacterial luciferase, FMN oxidoreductase and a protein, such as an antibody or an oligonucleotide coimmobilized on Sepharose, has been used to detect a label enzyme (Glucose 6 phosphate dehydrogenase). The label enzyme, bound to the solid phase, produces NADH and start an enzymatic chain reaction leading to light emission. The dehydrogenase, which is not bound to the solid phase, produces NADH in solution which is rapidly oxidized by a scavenger system (lactate dehydrogenase plus pyruvate) and thus does not participate in light emission. Using this solid phase, binding assays do not require separation of the excess of label, and the assay protocol is limited to the addition of sample, and luminescent reagents. The authors have used this solid phase for rapid immunoassays of haptens and proteins but also for the rapid quantitation of DNA sequences obtained by enzymatic amplification catalysed by a thermostable DNA polymerase.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008156 Luciferases Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates. Luciferase
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D009247 NADH, NADPH Oxidoreductases A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6. Oxidoreductases, NADH, NADPH,NADPH Oxidoreductases NADH,Oxidoreductases NADH, NADPH
D005954 Glucosephosphate Dehydrogenase Glucose-6-Phosphate Dehydrogenase,Dehydrogenase, Glucose-6-Phosphate,Dehydrogenase, Glucosephosphate,Glucose 6 Phosphate Dehydrogenase
D015336 Molecular Probe Techniques The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms. Molecular Probe Technic,Molecular Probe Technics,Molecular Probe Technique,Technic, Molecular Probe,Technics, Molecular Probe,Technique, Molecular Probe,Techniques, Molecular Probe,Probe Technic, Molecular,Probe Technics, Molecular,Probe Technique, Molecular,Probe Techniques, Molecular
D038181 FMN Reductase An enzyme that utilizes NADH or NADPH to reduce FLAVINS. It is involved in a number of biological processes that require reduced flavin for their functions such as bacterial bioluminescence. Formerly listed as EC 1.6.8.1 and EC 1.5.1.29. Flavin Mononucleotide Reductase,NAD(P)H-Flavin Oxidoreductase,FMN Oxidoreductase,NAD(P)H Dehydrogenase (FMN),NAD(P)H-FMN Oxidoreductase,NADH-FMN Oxidoreductase,NADH-Flavin Oxidoreductase,NADPH-Flavin Reductase,Mononucleotide Reductase, Flavin,NADH FMN Oxidoreductase,NADPH Flavin Reductase,Oxidoreductase, FMN,Oxidoreductase, NADH-FMN,Oxidoreductase, NADH-Flavin,Reductase, FMN,Reductase, Flavin Mononucleotide,Reductase, NADPH-Flavin

Related Publications

J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Bioluminescent stem-loop probes for highly sensitive nucleic acid detection.
September 2011, Chemical communications (Cambridge, England),
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Immobilization of microorganisms for detection by solid-phase immunoassays.
September 1985, Journal of clinical microbiology,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Solid-phase immunoassays.
January 2005, Current protocols in toxicology,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Bioluminescent Intercalating Dyes for Ratiometric Nucleic Acid Detection.
February 2024, ACS chemical biology,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
The use of tetramethylbenzidine for solid phase immunoassays.
December 1990, Journal of immunological methods,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Bioluminescent and chemiluminescent immunoassays.
January 1990, American clinical laboratory,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Chemiluminescent and bioluminescent immunoassays.
January 1987, Journal of pharmaceutical and biomedical analysis,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
A new plastic receptacle for solid phase immunoassays.
January 1978, Journal of immunological methods,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
ImmunoConcentration--a new format for solid-phase immunoassays.
September 1985, Clinical chemistry,
J C Nicolas, and B Térouanne, and P Balaguer, and A M Boussioux, and A Crastes de Paulet
Detection of antinuclear antibodies by solid-phase immunoassays and immunofluorescence analysis.
November 2004, Clinical chemistry,
Copied contents to your clipboard!