Perturbation theory in the catalytic rate constant of the Henri-Michaelis-Menten enzymatic reaction. 2012

Evangelos Bakalis, and Marios Kosmas, and Emmanouel M Papamichael
Dipartimento di Chimica "G. Ciamician", Universita di Bologna, Bologna, Italy. ebakalis@gmail.com

The Henry-Michaelis-Menten (HMM) mechanism of enzymatic reaction is studied by means of perturbation theory in the reaction rate constant k (2) of product formation. We present analytical solutions that provide the concentrations of the enzyme (E), the substrate (S), as well as those of the enzyme-substrate complex (C), and the product (P) as functions of time. For k (2) small compared to k (-1), we properly describe the entire enzymatic activity from the beginning of the reaction up to longer times without imposing extra conditions on the initial concentrations E ( o ) and S ( o ), which can be comparable or much different.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008956 Models, Chemical Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment. Chemical Models,Chemical Model,Model, Chemical
D009716 Numerical Analysis, Computer-Assisted Computer-assisted study of methods for obtaining useful quantitative solutions to problems that have been expressed mathematically. Analysis, Computer-Assisted Numerical,Computer-Assisted Numerical Analysis,Analyses, Computer-Assisted Numerical,Analysis, Computer Assisted Numerical,Computer Assisted Numerical Analysis,Computer-Assisted Numerical Analyses,Numerical Analyses, Computer-Assisted,Numerical Analysis, Computer Assisted
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D004798 Enzymes Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified. Biocatalyst,Enzyme,Biocatalysts

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