Biomaterial Database

A functional evaluation of cryopreserved peripheral nerve autografts. 1990

P A Ruwe, and T E Trumble

Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut.

This study compared the functional return following the repair of a 2.0-cm defect in the rat sciatic nerve with frozen (with or without treatment with a cryoprotectant, dimethyl sulfoxide [DMSO]) or fresh nerve grafts. In addition, a control group in which the defect was left ungrafted was evaluated. Recovery was assessed by functional studies (sensory testing, gait analysis, joint contractures, and tibialis anterior muscle weight), biochemical analysis (muscle hydroxyproline concentration), and histologic studies (axonal counts and fiber density). Sensory testing and gait analysis were not helpful because of cross-over innervation of the saphenous nerve and lack of recovery of the foot intrinsic muscles, respectively. The hind-limb contractures of the group with fresh nerve grafts (18 degrees +/- 3 degrees) were similar to those with DMSO-cryopreserved grafts (14 degrees +/- 4 degrees). Both of these groups had statistically smaller contractures than the non-DMSO-cryopreserved group (45 degrees +/- 8 degrees) and the gap control group (49 degrees +/- 6 degrees), (p less than 0.01). The tibialis anterior (TA) muscle-weight ratio (MWR) and the muscle-hydroxyproline-concentration ratio (MHPCR) showed a similar pattern, with no significant difference between the fresh (MWR, 0.5785 +/- 0.068 and MHPCR, 1.527 +/- 0.405) and DMSO-cryopreserved (MWR, 0.5675 +/- 0.0989 and MHPCR, 1.660 +/- 0.456) groups; both of these groups showed greater neurologic recovery when compared to the non-DMSO-cryopreserved (MWR, 0.3364 +/- 0.0266 and MHPCR, 3.441 +/- 0.300) and gap control (MWR, 0.2134 +/- 0.0775 and MHPCR, 4.869 +/- 2.351) groups.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008866	Microsurgery	The performance of surgical procedures with the aid of a microscope.
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009413	Nerve Fibers, Myelinated	A class of nerve fibers as defined by their structure, specifically the nerve sheath arrangement. The AXONS of the myelinated nerve fibers are completely encased in a MYELIN SHEATH. They are fibers of relatively large and varied diameters. Their NEURAL CONDUCTION rates are faster than those of the unmyelinated nerve fibers (NERVE FIBERS, UNMYELINATED). Myelinated nerve fibers are present in somatic and autonomic nerves.	A Fibers,B Fibers,Fiber, Myelinated Nerve,Fibers, Myelinated Nerve,Myelinated Nerve Fiber,Myelinated Nerve Fibers,Nerve Fiber, Myelinated
D009929	Organ Size	The measurement of an organ in volume, mass, or heaviness.	Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D011919	Rats, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.	August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D004121	Dimethyl Sulfoxide	A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.	DMSO,Dimethyl Sulphoxide,Dimethylsulfoxide,Dimethylsulphinyl,Dimethylsulphoxide,Dimexide,Rheumabene,Rimso,Rimso 100,Rimso-50,Sclerosol,Sulfinylbis(methane),Rimso 50,Rimso50,Sulfoxide, Dimethyl,Sulphoxide, Dimethyl
D005684	Gait	Manner or style of walking.	Gaits