Autofocusing of digital holographic microscopy based on off-axis illuminations. 2012

Peng Gao, and Baoli Yao, and Junwei Min, and Rongli Guo, and Baiheng Ma, and Juanjuan Zheng, and Ming Lei, and Shaohui Yan, and Dan Dan, and Tong Ye
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

An auto-focusing method for digital holographic microscopy has been proposed by employing two off-axis illumination beams. When specimens are illuminated by two plane waves in different directions, it is found that the farther the reconstruction plane is from the image plane, the wider the two reconstructed images are separated from each other. Thus, the image plane can be determinated by finding the minimum of the variation between the two reconstructed object waves on both the amplitude and phase distributions. The feasibility of the proposed method is demonstrated by the corresponding simulation and experiment.

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