Noninvasive imaging of portomesenteric venous anatomy in prospective pediatric liver transplant recipients may be limited by the small size of the vessels. A simple technique of percutaneous transhepatic portal venography was developed that involves the use of a 22-gauge needle and hand injection of contrast material. The technique was used in six infants (aged 4-15 months) with portal hypertension and end-stage liver disease due to biliary atresia. The portal vein was diagnostically opacified in four of the six patients. There were no complications related to the procedure.