Comparison of nine media in the culture of human ovarian granulosa lutein cells. 2012

Genovefa Bouraki, and Chryssa Metallinou, and Mara Simopoulou, and Konstantinos Charalabopoulos, and Byron Asimakopoulos
Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Thrace, Greece.

BACKGROUND Cultures of human ovarian granulosa lutein (hGL) cells are broadly used in experimental studies. The choice of the culture medium is important for the optimization of the conditions for culture of hGL cells. OBJECTIVE To compare the efficiency of a basic salt solution and eight different defined media on the culture of hGL cells. METHODS Cultures of the HGL-5 cell line were maintained for 72 hours with DMEM/F12, RPMI-1640, Ham'sF10, Modified Ham'sF10®, HTFXtra®, Global®, Complete Multiblast®, Universal® or Earle's balanced salt solution (EBSS). At the end of the culturing period, the attachment, the viability and the total number of cells were measured. RESULTS Culture in DMEM/F12 led to the highest score of all studied parameters, followed by RPMI-1640. The lowest performance was recorded with Complete Multiblast® and EBSS. The use of the other media gave mediocre results. CONCLUSIONS Among the media tested, DMEM/F12 appears to be the best choice for the culture of hGL cells.

UI MeSH Term Description Entries
D008184 Luteal Cells PROGESTERONE-producing cells in the CORPUS LUTEUM. The large luteal cells derive from the GRANULOSA CELLS. The small luteal cells derive from the THECA CELLS. Lutein Cells,Granulosa-Luteal Cells,Granulosa-Lutein Cells,Large Luteal Cells,Small Luteal Cells,Theca-Luteal cells,Theca-Lutein Cells,Cell, Granulosa-Luteal,Cell, Granulosa-Lutein,Cell, Large Luteal,Cell, Luteal,Cell, Lutein,Cell, Small Luteal,Cell, Theca-Lutein,Cells, Granulosa-Luteal,Cells, Granulosa-Lutein,Cells, Large Luteal,Cells, Luteal,Cells, Lutein,Cells, Small Luteal,Cells, Theca-Lutein,Granulosa Luteal Cells,Granulosa Lutein Cells,Granulosa-Luteal Cell,Granulosa-Lutein Cell,Large Luteal Cell,Luteal Cell,Luteal Cell, Large,Luteal Cell, Small,Luteal Cells, Large,Luteal Cells, Small,Lutein Cell,Small Luteal Cell,Theca Luteal cells,Theca Lutein Cells,Theca-Luteal cell,Theca-Lutein Cell,cell, Theca-Luteal,cells, Theca-Luteal
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005260 Female Females
D006107 Granulosa Cells Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH). Cell, Granulosa,Cells, Granulosa,Granulosa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D018929 Cell Culture Techniques Methods for maintaining or growing CELLS in vitro. Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell

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