The E3 ubiquitin ligase HOS1 regulates low ambient temperature-responsive flowering in Arabidopsis thaliana. 2012

Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
Creative Research Initiatives, Division of Life Sciences, Korea University, Seoul 136-701, Korea.

Ubiquitin-dependent proteolysis regulates multiple aspects of plant growth and development, but little is known about its role in ambient temperature-responsive flowering. In addition to being regulated by daylength, the onset of flowering in many plants can also be delayed by low ambient temperatures. Here, we show that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1), which encodes an E3 ubiquitin ligase, controls flowering time in response to ambient temperatures (16 and 23°C) and intermittent cold. hos1 mutants flowered early, and were insensitive to ambient temperature, but responded normally to vernalization and gibberellic acid. Genetic analyses suggested that this ambient temperature-insensitive flowering was independent of FLOWERING LOCUS C (FLC). Also, FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) expression was up-regulated in hos1 mutants at both temperatures. The ft tsf mutation almost completely suppressed the early flowering of hos1 mutants at different temperatures, suggesting that FT and TSF are downstream of HOS1 in the ambient temperature response. A lesion in CONSTANS (CO) did not affect the ambient temperature-insensitive flowering phenotype of hos1-3 mutants. In silico analysis showed that FVE was spatiotemporally co-expressed with HOS1. A HOS1-green fluorescent protein (GFP) fusion co-localized with FVE-GFP in the nucleus at both 16 and 23°C. HOS1 physically interacted with FVE and FLK in yeast two-hybrid and co-immunoprecipitation assays. Moreover, hos1 mutants were insensitive to intermittent cold. Collectively, our results suggest that HOS1 acts as a common regulator in the signaling pathways that control flowering time in response to low ambient temperature.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003080 Cold Temperature An absence of warmth or heat or a temperature notably below an accustomed norm. Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005875 Gibberellins A class of plant growth hormone isolated from cultures of GIBBERELLA FUJIKUROI, a fungus causing Bakanae disease in rice. There are many different members of the family as well as mixtures of multiple members; all are diterpenoid acids based on the gibberellane skeleton. Gibberellin
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription
D017360 Arabidopsis A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. Arabidopsis thaliana,Cress, Mouse-ear,A. thaliana,A. thalianas,Arabidopses,Arabidopsis thalianas,Cress, Mouse ear,Cresses, Mouse-ear,Mouse-ear Cress,Mouse-ear Cresses,thaliana, A.,thaliana, Arabidopsis,thalianas, A.
D047908 Intracellular Signaling Peptides and Proteins Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors. Intracellular Signaling Peptides,Intracellular Signaling Proteins,Peptides, Intracellular Signaling,Proteins, Intracellular Signaling,Signaling Peptides, Intracellular,Signaling Proteins, Intracellular
D049488 Phosphatidylethanolamine Binding Protein A ubiquitously found basic protein that binds to phosphatidylethanolamine and NUCLEOTIDES. It is an endogenous inhibitor of RAF KINASES and may play a role in regulating SIGNAL TRANSDUCTION. Phosphatidylethanolamine-binding protein is the precursor of hippocampal cholinergic neurostimulating peptide, which is cleaved from the N-terminal region of the protein. HCNP Precursor Protein,Hippocampal Cholinergic Neurostimulating Peptide Precursor,Phosphatidylethanolamine-Binding Protein,Raf Kinase Inhibitor Protein

Related Publications

Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
December 2012, The Journal of biological chemistry,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
March 2012, The Plant cell,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
September 2015, The Plant cell,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
September 2018, Plant cell reports,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
July 2015, Scientific reports,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
January 2015, Plant signaling & behavior,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
July 2018, BMC plant biology,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
November 2016, Proceedings of the National Academy of Sciences of the United States of America,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
January 2021, Frontiers in plant science,
Jeong Hwan Lee, and Jae Joon Kim, and Soo Hyun Kim, and Hyun Jung Cho, and Joonki Kim, and Ji Hoon Ahn
January 2016, Journal of integrative plant biology,
Copied contents to your clipboard!