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Amyloid peptides are important components in many degenerative diseases as well as in maintaining cellular metabolism. Their unique stable structure provides new insights in developing new materials. Designing bioinspired self-assembling peptides is essential to generate new forms of hierarchical nanostructures. Here we present oppositely charged amyloid inspired peptides (AIPs), which rapidly self-assemble into nanofibers at pH 7 upon mixing in water caused by noncovalent interactions. Mechanical properties of the gels formed by self-assembled AIP nanofibers were analyzed with oscillatory rheology. AIP gels exhibited strong mechanical characteristics superior to gels formed by self-assembly of previously reported synthetic short peptides. Rheological studies of gels composed of oppositely charged mixed AIP molecules (AIP-1 + 2) revealed superior mechanical stability compared to individual peptide networks (AIP-1 and AIP-2) formed by neutralization of net charges through pH change. Adhesion and elasticity properties of AIP mixed nanofibers and charge neutralized AIP-1, AIP-2 nanofibers were analyzed by high resolution force-distance mapping using atomic force microscopy (AFM). Nanomechanical characterization of self-assembled AIP-1 + 2, AIP-1, and AIP-2 nanofibers also confirmed macroscopic rheology results, and mechanical stability of AIP mixed nanofibers was higher compared to individual AIP-1 and AIP-2 nanofibers self-assembled at acidic and basic pH, respectively. Experimental results were supported with molecular dynamics simulations by considering potential noncovalent interactions between the amino acid residues and possible aggregate forms. In addition, HUVEC cells were cultured on AIP mixed nanofibers at pH 7 and biocompatibility and collagen mimetic scaffold properties of the nanofibrous system were observed. Encapsulation of a zwitterionic dye (rhodamine B) within AIP nanofiber network was accomplished at physiological conditions to demonstrate that this network can be utilized for inclusion of soluble factors as a scaffold for cell culture studies.
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
November 2022,
The journal of physical chemistry. B,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
March 2014,
Acta biomaterialia,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
August 2021,
Biomaterials science,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
January 2021,
ACS applied materials & interfaces,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
March 2007,
Soft matter,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
January 2005,
Nano letters,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
May 2013,
Biomacromolecules,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
January 2006,
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
February 2006,
Soft matter,
Goksu Cinar, and
Hakan Ceylan, and
Mustafa Urel, and
Turan S Erkal, and
E Deniz Tekin, and
Ayse B Tekinay, and
Aykutlu Dâna, and
Mustafa O Guler
April 2020,
Nanoscale,
