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A novel endogeneous inhibitor from hepatopancreas of Kamchatka crab (Paralithosed camtschaticus) was isolatyed. The inhibitor was purifeid through fractional affinity chromatography on gramicidin-diasorb followed by gel-filtration at Sephadex G-100. The inhibitor PC is a protein (M, 66 kDa) and active against serine collagenolytic protease PC at temperature optimum 15-20 degrees C, stable at 4-40 degrees C and was completely inactivated after heating to 50 degrees C and higher. 0.9-20% NaCl is necessary for its inhibitor activity. The inhibitor was found to slow down cell spreading in vitro in cell type-dependent manner. Fibroblasts are most prone to inhibitory effect, epithelial tumor derived cells show medium susceptibility, while fibrosarcoma cells were not affected.
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Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
January 2003,
Prikladnaia biokhimiia i mikrobiologiia,
S A Kuznetsov, and
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Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
February 2000,
Biochemistry. Biokhimiia,
S A Kuznetsov, and
S A Golyshev, and
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I P Kuranova, and
G N Rudenskaia
January 2008,
Prikladnaia biokhimiia i mikrobiologiia,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
January 2007,
Prikladnaia biokhimiia i mikrobiologiia,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
August 2000,
Genetika,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
October 2018,
Fish & shellfish immunology,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
April 1995,
Bioorganicheskaia khimiia,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
January 2003,
Bioorganicheskaia khimiia,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
January 2009,
Prikladnaia biokhimiia i mikrobiologiia,
S A Kuznetsov, and
S A Golyshev, and
Iu A Rudenskaia, and
V A Isaev, and
I P Kuranova, and
G N Rudenskaia
January 2018,
Journal of natural products,
