Biomaterial Database

Photosystem I in Langmuir-Blodgett and Langmuir-Schaefer monolayers. 2012

Xun Yan, and Christopher J Faulkner, and G Kane Jennings, and David E Cliffel

Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235-1822, United States.

Photosystem I (PSI) is a membrane protein complex that generates photoinduced electrons and transfers them across the thylakoid membrane during photosynthesis. The PSI complex, separated from spinach leaves, was spread onto the air-water interface as a monolayer and transferred onto a gold electrode surface that was precoated with a self-assembled monolayer (SAM). The electrochemical properties of the transferred PSI monolayer, including cyclic voltammetry and photoinduced chronoamperometry, were measured. The results showed that PSI retained its bioactivity after the manipulation. Its capability of converting photoenergy into electrical potential was demonstrated by its reducing an electron acceptor, dichloroindophenol (DCIP), and by oxidizing an electron donor, sodium ascorbate (ASC). We have shown that the protein has two possible orientations at the water interface. The orientation distribution was determined by comparing the controlled reductive and oxidative photocurrents generated from Langmuir-Blodgett and Langmuir-Schaefer monolayers.

UI	MeSH Term	Description	Entries
D004566	Electrodes	Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.	Anode,Anode Materials,Cathode,Cathode Materials,Anode Material,Anodes,Cathode Material,Cathodes,Electrode,Material, Anode,Material, Cathode
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D000388	Air	The mixture of gases present in the earth's atmosphere consisting of oxygen, nitrogen, carbon dioxide, and small amounts of other gases.
D001205	Ascorbic Acid	A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.	Vitamin C,Ascorbic Acid, Monosodium Salt,Ferrous Ascorbate,Hybrin,L-Ascorbic Acid,Magnesium Ascorbate,Magnesium Ascorbicum,Magnesium di-L-Ascorbate,Magnorbin,Sodium Ascorbate,Acid, Ascorbic,Acid, L-Ascorbic,Ascorbate, Ferrous,Ascorbate, Magnesium,Ascorbate, Sodium,L Ascorbic Acid,Magnesium di L Ascorbate,di-L-Ascorbate, Magnesium
D013499	Surface Properties	Characteristics or attributes of the outer boundaries of objects, including molecules.	Properties, Surface,Property, Surface,Surface Property
D014867	Water	A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Hydrogen Oxide
D015086	2,6-Dichloroindophenol	A dye used as a reagent in the determination of vitamin C.	2,6-Dichlorophenolindophenol,Sodium 2,6-Dichloroindophenol,Tillmans' Reagent,2,6 Dichloroindophenol,2,6-Dichlorobenzenoneindophenol Dye,Dichlorophenol Indophenol,Dichlorophenolindophenol,Tillmans Reagent,2,6 Dichlorobenzenoneindophenol Dye,2,6 Dichlorophenolindophenol,2,6-Dichloroindophenol, Sodium,Dichloroindophenol, 2,6,Dye, 2,6-Dichlorobenzenoneindophenol,Indophenol, Dichlorophenol,Reagent, Tillmans,Reagent, Tillmans',Sodium 2,6 Dichloroindophenol,Tillman Reagent,Tillman's Reagent
D045331	Photosystem I Protein Complex	A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.	Photosystem I Reaction Center,Photosystem I
D055664	Electrochemical Techniques	The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.	Electrochemical Technics,Electrochemical Technic,Electrochemical Technique,Technic, Electrochemical,Technics, Electrochemical,Technique, Electrochemical,Techniques, Electrochemical