-transparent.png){kind=logo}
Internal amino acid sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels. 1990
Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research, (Melbourne Branch), Parkville, Victoria, Australia.
A new method was developed for generating peptide fragments for amino acid sequence analysis from polyacrylamide-gel separated proteins. This method involves in situ CNBr treatment of proteins in the polyacrylamide gel after their separation by electrophoresis. Pure CNBr peptides were recovered either by solvent extraction followed by microbore column reversed-phase HPLC or, alternatively, by a second electrophoretic separation step (SDS-PAGE) followed by electrotransfer of the peptides onto polyvinylidene difluoride (PVDF) membranes. These approaches yielded sequence data at subnanomole levels for a wide range of CNBr fragments recovered from gel-separated proteins.
Related Publications
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
September 1988,
Biochemical and biophysical research communications,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
September 1981,
The Biochemical journal,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
August 1997,
Journal of biochemical and biophysical methods,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
November 1976,
Analytical biochemistry,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
February 1989,
Analytical biochemistry,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
January 1976,
Analytical biochemistry,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
July 1983,
Analytical biochemistry,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
July 1970,
The Journal of biological chemistry,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
May 1972,
Biochimica et biophysica acta,
W Jahnen, and
L D Ward, and
G E Reid, and
R L Moritz, and
R J Simpson
January 1983,
Methods in enzymology,
