Kinetic studies of L-asparaginase from Penicillium digitatum. 2012

Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
Department of Biotechnology, College of Life Sciences, Cancer Hospital & Research Institute, Gwalior-MP, 474009 India. abhi.shri76@gmail.com

L-Asparaginase is an enzyme used in the treatment of acute lymphoblastic leukemia and other related malignancies. Its further use includes reduction of asparagine concentration in food products, which may lead to formation of acrylamide. Currently bacterial asparaginase is produced at industrial scale, but the enzyme isolated from bacterial origin is often associated with adverse reactions. These side effects require development of asparaginase from alternative sources. In the present study, Penicillium digitatum was explored for the production of extracellular L-asparaginase using modified Czapek-Dox media. The enzyme was purified about 60.95-fold and then kinetic study showed that the Km value of the enzyme was 1 × 10⁻⁵ M. The optimum pH and temperature for the enzyme were 7.0 and 30°C, respectively. The optimum incubation period for L-asparaginase was 15 min. This work concludes that this enzyme can be a suitable candidate due to its strong kinetic properties, and further research can usher into development of asparaginase formulation from fungal origin with less adverse effects.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010407 Penicillium A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin. Penicilliums
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001215 Asparaginase A hydrolase enzyme that converts L-asparagine and water to L-aspartate and NH3. EC 3.5.1.1. Asparaginase II,Asparaginase medac,Asparagine Deaminase,Colaspase,Crasnitin,Elspar,Erwinase,Kidrolase,Leunase,Paronal,Deaminase, Asparagine,medac, Asparaginase
D001216 Asparagine A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed) L-Asparagine
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
March 1986, Biotechnology progress,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
February 1973, Journal of applied physiology,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
February 1959, Canadian journal of microbiology,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
June 2022, Pharmaceuticals (Basel, Switzerland),
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
December 1965, Nature,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
November 1969, Nature,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
December 1971, Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
October 2011, Biotechnology letters,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
January 1988, Drug metabolism and disposition: the biological fate of chemicals,
Abhinav Shrivastava, and Abdul Arif Khan, and Archana Shrivastav, and Sudhir K Jain, and Pradeep K Singhal
July 2016, Virology,
Copied contents to your clipboard!