Biomaterial Database

Developmental tissue expression and phylogenetic conservation of stathmin, a phosphoprotein associated with cell regulations. 1990

J Koppel, and M C Boutterin, and V Doye, and H Peyro-Saint-Paul, and A Sobel

Institut National de la Santé et de la Recherche Médicale U 153, Centre National de la Recherche Scientifique URA 614, Paris, France.

Stathmin is a 19-kDa phosphoprotein presumably involved in regulations of cell proliferation, differentiation, and functions as an intracellular relay for extracellular signals activating diverse second messenger pathways. Antisera prepared against the whole protein or against two peptides (residues 15-27 and 134-149) recognized the two isoforms (alpha and beta) of stathmin in their different phosphorylated states on immunoblots. Also, the possible existence of a family of stathmin-related proteins is suggested by the detection with some sera of proteins of 17, 21, and 60 kDa in brain. Stathmin and its diverse molecular forms were detected in all mouse tissues tested, in varying concentrations. Depending on the tissue, it is 2-100 times more abundant in the neonate than in the adult. It is most abundant in brain at both developmental stages, the protein levels being paralleled by the expression of the corresponding mRNA as detected with a specific cDNA probe. Antibodies directed against the rat protein also reacted with stathmin-like proteins in the brain of other mammals, birds, reptiles, amphibians, and some fish species, and the various isoforms could be recognized on immunoblots. In conclusion, our results suggest that stathmin is most likely involved in two distinct types of regulations: 1) "developmental" regulations, related to cell proliferation, differentiation, and maturation, and 2) "functional" regulations mostly at the adult stage, and typically in the nervous system. In addition, stathmin is also phylogenetically well conserved at least in vertebrates. Together, these observations support the proposed ubiquitous nature and general importance of stathmin in biological regulations.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D008116	Lizards	Reptiles within the order Squamata that generally possess limbs, moveable EYELIDS, and EXTERNAL EAR openings, although there are some species which lack one or more of these structures.	Chameleons,Geckos,Chameleon,Gecko,Lizard
D008297	Male		Males
D008868	Microtubule Proteins	Proteins found in the microtubules.	Proteins, Microtubule
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010750	Phosphoproteins		Phosphoprotein
D010802	Phylogeny	The relationships of groups of organisms as reflected by their genetic makeup.	Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011898	Ranidae	The family of true frogs of the order Anura. The family occurs worldwide except in Antarctica.	Frogs, True,Rana,Frog, True,True Frog,True Frogs