Biomaterial Database

Collagen production in diabetic wounded fibroblasts in response to low-intensity laser irradiation at 660 nm. 2012

Sandra M Ayuk, and Nicolette N Houreld, and Heidi Abrahamse

Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa.

BACKGROUND Collagen type I (Col-I) is a major component of the extracellular matrix and is important in wound healing processes. Several studies have shown that low-intensity laser irradiation (LILI) biostimulates Col-I synthesis both in vitro and in vivo. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model. METHODS This study was performed on isolated human skin fibroblasts. Different cell models (normal and diabetic wounded) were used. Cells were irradiated with 5 J/cm(2) at a wavelength of 660 nm and incubated for 48 or 72 h. Nonirradiated cells (0 J/cm(2)) were used as controls. Cellular viability (Trypan blue exclusion test), morphology (bright-field microscopy), proliferation [VisionBlue™ quick cell proliferation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and Col-I (enzyme-linked immunoabsorbent assay) were assessed. RESULTS Diabetic wounded cells irradiated with 5 J/cm(2) at 660 nm showed a significant increase in cell migration, viability, proliferation, and collagen content. CONCLUSIONS This study shows that LILI stimulates Col-I synthesis in diabetic wound healing in vitro at 660 nm.

UI	MeSH Term	Description	Entries
D011829	Radiation Dosage	The amount of radiation energy that is deposited in a unit mass of material, such as tissues of plants or animal. In RADIOTHERAPY, radiation dosage is expressed in gray units (Gy). In RADIOLOGIC HEALTH, the dosage is expressed by the product of absorbed dose (Gy) and quality factor (a function of linear energy transfer), and is called radiation dose equivalent in sievert units (Sv).	Sievert Units,Dosage, Radiation,Gray Units,Gy Radiation,Sv Radiation Dose Equivalent,Dosages, Radiation,Radiation Dosages,Units, Gray,Units, Sievert
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003094	Collagen	A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).	Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D014945	Wound Healing	Restoration of integrity to traumatized tissue.	Healing, Wound,Healings, Wound,Wound Healings
D017719	Diabetic Foot	Common foot problems in persons with DIABETES MELLITUS, caused by any combination of factors such as DIABETIC NEUROPATHIES; PERIPHERAL VASCULAR DISEASES; and INFECTION. With the loss of sensation and poor circulation, injuries and infections often lead to severe foot ulceration, GANGRENE and AMPUTATION, SURGICAL.	Foot Ulcer, Diabetic,Diabetic Feet,Feet, Diabetic,Foot, Diabetic
D049109	Cell Proliferation	All of the processes involved in increasing CELL NUMBER including CELL DIVISION.	Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D028022	Low-Level Light Therapy	Treatment using irradiation with light of low power intensity so that the effects are a response to the light and not due to heat. A variety of light sources, especially low-power lasers are used.	LLLT,Laser Biostimulation,Laser Irradiation, Low-Power,Laser Therapy, Low-Level,Photobiomodulation,Laser Phototherapy,Laser Therapy, Low-Power,Low-Level Laser Therapy,Low-Power Laser Irradiation,Low-Power Laser Therapy,Photobiomodulation Therapy,Biostimulation, Laser,Irradiation, Low-Power Laser,Laser Irradiation, Low Power,Laser Therapies, Low-Level,Laser Therapies, Low-Power,Laser Therapy, Low Level,Laser Therapy, Low Power,Light Therapies, Low-Level,Light Therapy, Low-Level,Low Level Laser Therapy,Low Level Light Therapy,Low Power Laser Irradiation,Low Power Laser Therapy,Low-Level Laser Therapies,Low-Level Light Therapies,Low-Power Laser Therapies,Photobiomodulation Therapies,Photobiomodulations,Phototherapy, Laser,Therapies, Low-Level Light,Therapies, Photobiomodulation,Therapy, Low-Level Light,Therapy, Photobiomodulation