BACKGROUND This study aims to investigate the effects of tropicamide 1% on the main numerical parameters of anterior segment with the Pentacam rotating Scheimpflug camera in healthy individuals. METHODS Pentacam measurements of 50 (25 female and 25 male) healthy individuals before and after 25 min of tropicamide 1% instillation were performed and compared. For comparison of the test parameters, paired t-test was used in the groups. RESULTS Mean age in the study group was 28.40±4.58 years (range 18-35 years). Measurements between 2 sessions were significantly different for the parameters of anterior chamber depth (ACD) and anterior chamber volume (ACV) (P<0.05). CONCLUSIONS Tropicamide 1% instillation leads to a significant increment in ACD and ACV measurements. Effects of tropicamide on these parameters for Pentacam measurements should be considered to ensure exact clinical interpretation in ophthalmologic practice.