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The rate equation for a tight-binding inhibitor of an enzyme-catalysed first-order reversible reaction was used to derive two integrated equations. One of them covers the situations in which competitive, uncompetitive or non-competitive inhibition occurs and the other refers to the special non-competitive case where the two inhibition constants are equal. For these equations, graphical and non-linear regression methods are proposed for distinguishing between types of inhibition and for calculating inhibition constants from progress-curve data. The application of the non-linear regression to the analysis of stimulated progress curves in the presence of a tight-binding inhibitor is also presented. The results obtained are valid for any type of 'dead-end'-complex-forming inhibitor and can be used to characterize an unknown inhibitor on the basis of progress curves.
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
January 1969,
Biochimica et biophysica acta,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
December 1994,
Biochimica et biophysica acta,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
March 1989,
The Biochemical journal,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
October 1984,
The Biochemical journal,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
April 1977,
Biochimica et biophysica acta,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
February 1985,
The Biochemical journal,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
January 1982,
Molekuliarnaia biologiia,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
October 1975,
International journal of bio-medical computing,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
March 1974,
Journal of theoretical biology,
S E Szedlacsek, and
V Ostafe, and
R G Duggleby, and
M Serban, and
M O Vlad
January 1986,
Advances in experimental medicine and biology,
