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Immunoglobulins from antiserum raised against chromatographically purified avian myeloblastosis virus (AMV) group-specific (gs) antigens were used in enzyme-linked immunosorbent assay (ELISA). Readily discernible color was produced with 2--3 ng of AMV protein in microplate wells coated with 4 micrograms of salt-precipitated immunoglobulins. When a biological assay, i.e., phenotypic mixing (PM), was the criterion for the infectious status of specimens, the ELISA consistently identified a greater percentage of virus-positive specimens than direct complement-fixation (DCF) tests. Over 95% concordance was obtained between the ELISA and PM bioassays when meconia and whole-blood samples were tested. Moreover, three DCF(-) egg albumens from one virus shedder hen were positive by the direct ELISA. Complete agreement was found between a biological assay for endogenous virus and the ELISA when blood and albumens from inbred chickens were tested. The ELISA is a rapid and convenient alternative to the DCF test for identifying infected chickens in eradication programs, because virus-rich sources such as meconia and blood that are unsuitable for DCF can be tested directly.
E J Smith, and
A Fadly, and
W Okazaki
March 1986,
American journal of veterinary research,
E J Smith, and
A Fadly, and
W Okazaki
January 1985,
Avian diseases,
E J Smith, and
A Fadly, and
W Okazaki
June 2012,
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi,
E J Smith, and
A Fadly, and
W Okazaki
April 2022,
Journal of virological methods,
E J Smith, and
A Fadly, and
W Okazaki
August 1980,
The Veterinary record,
E J Smith, and
A Fadly, and
W Okazaki
January 1984,
Hepatology (Baltimore, Md.),
E J Smith, and
A Fadly, and
W Okazaki
March 1984,
Poultry science,
E J Smith, and
A Fadly, and
W Okazaki
January 1986,
Folia biologica,
E J Smith, and
A Fadly, and
W Okazaki
January 1979,
Transfusion,
E J Smith, and
A Fadly, and
W Okazaki
November 1970,
Avian diseases,
