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Human large bowel lamina propria lymphoid cells have been isolated using both mechanical and enzymatic techniques. Their separation from other cell types after isolation was effected with greater efficiency by sedimentation on isokinetic gradients than by filtration through glass bead columns. After being purified, the capacity of the lamina propria lymphocytes to function in vitro as effector cells in antibody-dependent cellular cytotoxicity was determined. Mechanical distruption of the mucosa gave low yields of lymphoid cells, which lacked the capacity for cytotoxicity. Enzymatic digestion of mucosal tissue, by comparison, yielded large numbers of viable lymphoid cells which retained a significant level of cytotoxic activity. Investigation revealed that mechanical homogenisation stimulated the synthesis of prostaglandin E2, and inhibitor studies showed that this mediator was responsible for the lack of cytotoxic activity in mechanically-liberated lymphocytes.
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
May 1977,
The Journal of clinical investigation,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
September 1979,
Gastroenterology,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
February 1979,
Gastroenterology,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
April 1981,
Transplantation,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
June 1982,
Journal of immunology (Baltimore, Md. : 1950),
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
August 2018,
Current protocols in immunology,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
April 1984,
The American journal of physiology,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
January 1982,
Advances in experimental medicine and biology,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
August 1982,
Tsitologiia,
P W Bland, and
E R Richens, and
D C Britton, and
J V Lloyd
September 1979,
Digestive diseases and sciences,
