An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y. 2012

Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brasil. alexsantos@ioc.fiocruz.br

The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our "in-house" assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed "in house" could be used as an alternative to commercial assays that utilise IgG.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D007136 Immunoglobulins Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses. Globulins, Immune,Immune Globulin,Immune Globulins,Immunoglobulin,Globulin, Immune
D006506 Hepatitis A INFLAMMATION of the LIVER in humans caused by a member of the HEPATOVIRUS genus, HUMAN HEPATITIS A VIRUS. It can be transmitted through fecal contamination of food or water. Hepatitis, Infectious,Infectious Hepatitis,Hepatitides, Infectious,Infectious Hepatitides
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D030041 Hepatitis A virus A species in the genus HEPATOVIRUS containing one serotype and two strains: HUMAN HEPATITIS A VIRUS and Simian hepatitis A virus causing hepatitis in humans (HEPATITIS A) and primates, respectively. Hepatitis A viruses
D035922 Hepatitis A Antibodies Antibodies to the HEPATITIS A ANTIGENS including antibodies to envelope, core, and non-structural proteins. Hepatitis A Virus Antibodies,Anti-HAV,Anti HAV,Antibodies, Hepatitis A

Related Publications

Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
January 1990, Methods in enzymology,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
March 1984, Journal of immunological methods,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
February 2005, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
September 1992, Journal of clinical microbiology,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
August 1982, Journal of clinical microbiology,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
July 2016, The American journal of tropical medicine and hygiene,
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
December 1987, Diabetes research (Edinburgh, Scotland),
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
April 1974, Lancet (London, England),
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
April 1975, Lancet (London, England),
Alexandre dos Santos da Silva, and Gentil Arthur Lins Bentes Mendonça de Vasconcelos, and Livia Abud Kappel, and Marcelo Alves Pinto, and Vanessa Salete de Paula
April 1986, Clinical chemistry,
Copied contents to your clipboard!