Biomaterial Database

Natural biofilm formation with Legionella pneumophila. 2013

Emilie Portier, and Yann Héchard

Faculté de Pharmacie, Laboratoire de Génie Chimique, BioSym Department, Université de Toulouse, Toulouse, France.

Biofilm formation could be studied in various conditions. Most of the studies with Legionella pneumophila used monospecies biofilm in culture media. In some cases, it is important to study bacteria in conditions more close to environmental conditions. In this paper, we describe protocols to produce natural biofilms from river water that were spiked with L. pneumophila.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005618	Fresh Water	Water containing no significant amounts of salts, such as water from RIVERS and LAKES.	Freshwater,Fresh Waters,Freshwaters,Water, Fresh,Waters, Fresh
D014871	Water Microbiology	The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.	Microbiology, Water
D016952	Legionella pneumophila	A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.
D018441	Biofilms	Encrustations formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedded in an EXTRACELLULAR POLYMERIC SUBSTANCE MATRIX that is secreted by the microbes. They occur on body surfaces such as teeth (DENTAL DEPOSITS); inanimate objects, and bodies of water. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and anti-fouling agents.	Biofilm