-transparent.png){kind=logo}
The effect of adding either reduced (GSH) or oxidized (GSSG) glutathione to intact human erythrocytes was investigated by 1H-spin echo NMR, which allows direct observation of relatively concentrated low molecular weight compounds within intact cells. A specific region of the spectrum was affected by addition of GSH, with the appearance of new peaks that were diagnostic of an increase of intracellular GSH. These changes did not occur in hemolysates, and did not involve extra-cytosol GSH either free or membrane-bound. These results indicate that the intracellular redox balance of glutathione is shifted toward the reduced state by exogenous glutathione, possibly via a signal transferring system of the cell membrane.
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
November 1980,
FEBS letters,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
September 1991,
Clinica chimica acta; international journal of clinical chemistry,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
October 1979,
FEBS letters,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
August 1986,
Biochimica et biophysica acta,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
December 1990,
NMR in biomedicine,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
April 1982,
Analytical biochemistry,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
October 1977,
FEBS letters,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
March 1993,
European journal of biochemistry,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
February 1988,
Magnetic resonance in medicine,
M R Ciriolo, and
M Sette, and
M Paci, and
G Rotilio
May 1992,
Journal of medicinal chemistry,
