BIOMAT Database Logo BIOMAT Database Logo

Evaluation of the VITEK 2 gram positive (GP) microbial identification test card: collaborative study. 2012

Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA.

A collaborative study was conducted to evaluate the performance of the VITEK 2 Gram Positive (GP) identification card for use with the VITEK 2 automated microbial identification system. The GP test card is used in the identification of selected Gram positive organisms, including Listeria and Staphylococcus species. The VITEK 2 GP card is based on 43 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing laboratories throughout the United States participated. In this study, 720 Gram-positive inclusivity isolates were analyzed by the GP Identification method. Of the 720 well-characterized isolates, 714 were identified correctly, zero were misidentified, zero were unidentified, and six were not characterized as a Gram-positive organism by the VITEK 2 GP method. Additionally, 120 strains exclusive of Gram-positive organisms were screened by Gram stain. A total of 106 isolates were correctly excluded. Fourteen organisms were incorrectly characterized by Gram stain procedures, thus resulting in improper analysis and misidentification by VITEK GP. The VITEK 2 GP identification method is an acceptable automated method for the rapid identification of selected Gram-positive bacteria.

UI MeSH Term Description Entries
D007753 Laboratories Facilities equipped to carry out investigative procedures. Laboratory
D011933 Reagent Kits, Diagnostic Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use. Diagnostic Reagent Kits,Diagnostic Reagents and Test Kits,Diagnostic Test Kits,In Vitro Diagnostic Device,In Vitro Diagnostic Devices,In Vitro Diagnostic Medical Device,In Vitro Diagnostic Medical Devices,Kits, Diagnostic Reagent,Diagnostic Reagent Kit,Diagnostic Test Kit,Kit, Diagnostic Reagent,Kit, Diagnostic Test,Kits, Diagnostic Test,Reagent Kit, Diagnostic,Test Kit, Diagnostic,Test Kits, Diagnostic
D006094 Gram-Positive Bacteria Bacteria which retain the crystal violet stain when treated by Gram's method. Gram Positive Bacteria
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D016908 Gram-Positive Bacterial Infections Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method. Bacterial Infections, Gram-Positive,Infections, Gram-Positive Bacterial,Bacterial Infection, Gram-Positive,Bacterial Infections, Gram Positive,Gram Positive Bacterial Infections,Gram-Positive Bacterial Infection,Infection, Gram-Positive Bacterial,Infections, Gram Positive Bacterial

Related Publications

Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Evaluation of the Vitek Systems Gram-Positive Identification card for species identification of coagulase-negative staphylococci.
May 1993, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Performance of the new VITEK 2 GP card for identification of medically relevant gram-positive cocci in a routine clinical laboratory.
January 2005, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Evaluation of the new VITEK 2 card for identification of clinically relevant gram-negative rods.
September 2004, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Microbiological evaluation of the new VITEK 2 Neisseria-Haemophilus identification card.
November 2007, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card.
August 2008, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Multicenter evaluation of the Vitek 2 anaerobe and Corynebacterium identification card.
August 2008, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
New Vitek 2 colorimetric GN Card for identification of gram-negative nonfermentative bacilli.
December 2007, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.
April 2007, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Clinical evaluation of the Vitek Neisseria-Haemophilus Identification card.
January 1987, Journal of clinical microbiology,
Erin Crowley, and Patrick Bird, and Kiel Fisher, and Katherine Goetz, and Megan Boyle, and M Joseph Benzinger, and Marc Juenger, and James Agin, and David Goins, and Ronald L Johnson
Evaluation of the new Vitek 2 GN card for the identification of gram-negative bacilli frequently encountered in clinical laboratories.
October 2005, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
Copied contents to your clipboard!