The role of drebrin in glioma migration and invasion. 2013

Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario, Canada.

Glioblastoma (GBM) is the most common primary brain tumor in adults. Despite current advances in therapy consisting of surgery followed by chemotherapy and radiation, the overall survival rate still remains poor. Therapeutic failures are partly attributable to the highly infiltrative nature of tumor adjacent to normal brain parenchyma. Recently, evidence is mounting to suggest that actin cytoskeleton dynamics are critical components of the cell invasion process. Drebrin is an actin-binding protein involved in the regulation of actin filament organization, and plays a significant role in cell motility; however, the role of drebrin in glioma cell invasiveness has not yet been fully elucidated. Therefore, this study was aimed to clarify the role of drebrin in glioma cell morphology and cell motility. Here we show that drebrin is expressed in glioma cell lines and in operative specimens of GBM. We demonstrate that stable overexpression of drebrin in U87 cells leads to alterations in cell morphology, and induces increased invasiveness in vitro while knockdown of drebrin in U87 cells by small interfering RNA (siRNA) decreases invasion and migration. In addition, we show that depletion of drebrin by siRNA alters glioma cell morphology in A172 GBM cell line. Our results suggest that drebrin contributes to the maintenance of cell shape, and may play an important role in glioma cell motility.

UI MeSH Term Description Entries
D009361 Neoplasm Invasiveness Ability of neoplasms to infiltrate and actively destroy surrounding tissue. Invasiveness, Neoplasm,Neoplasm Invasion,Invasion, Neoplasm
D009479 Neuropeptides Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells. Neuropeptide
D001932 Brain Neoplasms Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain. Brain Cancer,Brain Metastases,Brain Tumors,Cancer of Brain,Malignant Primary Brain Tumors,Neoplasms, Intracranial,Benign Neoplasms, Brain,Brain Neoplasm, Primary,Brain Neoplasms, Benign,Brain Neoplasms, Malignant,Brain Neoplasms, Malignant, Primary,Brain Neoplasms, Primary Malignant,Brain Tumor, Primary,Brain Tumor, Recurrent,Cancer of the Brain,Intracranial Neoplasms,Malignant Neoplasms, Brain,Malignant Primary Brain Neoplasms,Neoplasms, Brain,Neoplasms, Brain, Benign,Neoplasms, Brain, Malignant,Neoplasms, Brain, Primary,Primary Brain Neoplasms,Primary Malignant Brain Neoplasms,Primary Malignant Brain Tumors,Benign Brain Neoplasm,Benign Brain Neoplasms,Benign Neoplasm, Brain,Brain Benign Neoplasm,Brain Benign Neoplasms,Brain Cancers,Brain Malignant Neoplasm,Brain Malignant Neoplasms,Brain Metastase,Brain Neoplasm,Brain Neoplasm, Benign,Brain Neoplasm, Malignant,Brain Neoplasms, Primary,Brain Tumor,Brain Tumors, Recurrent,Cancer, Brain,Intracranial Neoplasm,Malignant Brain Neoplasm,Malignant Brain Neoplasms,Malignant Neoplasm, Brain,Neoplasm, Brain,Neoplasm, Intracranial,Primary Brain Neoplasm,Primary Brain Tumor,Primary Brain Tumors,Recurrent Brain Tumor,Recurrent Brain Tumors,Tumor, Brain
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005910 Glioma Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21) Glial Cell Tumors,Malignant Glioma,Mixed Glioma,Glial Cell Tumor,Glioma, Malignant,Glioma, Mixed,Gliomas,Gliomas, Malignant,Gliomas, Mixed,Malignant Gliomas,Mixed Gliomas,Tumor, Glial Cell,Tumors, Glial Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines

Related Publications

Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
January 2017, Advances in experimental medicine and biology,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
November 2004, Cancer research,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
January 2013, World neurosurgery,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
January 2019, OncoTargets and therapy,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
October 2012, International journal of oncology,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
June 2011, International journal of oncology,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
December 2010, Neuroscience bulletin,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
March 2013, Anatomical record (Hoboken, N.J. : 2007),
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
November 2004, Journal of neuro-oncology,
Yuzo Terakawa, and Sameer Agnihotri, and Brian Golbourn, and Mustafa Nadi, and Nesrin Sabha, and Christian A Smith, and Sidney E Croul, and James T Rutka
June 2001, Journal of neurosurgery,
Copied contents to your clipboard!