BIOMAT Database Logo BIOMAT Database Logo

Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. 2013

Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, China.

An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) employing monoclonal and polyclonal antibodies against p27 was developed for the detection of the avian leukosis virus (ALV). The specificity of the optimized AC-ELISA was evaluated using avian leukosis virus subgroup J (ALV-J), avian leukosis virus subgroup A (ALV-A), avian leukosis virus subgroup B (ALV-B), avian infectious bronchitis virus (IBV), Marek's disease virus (MDV), avian infectious laryngotracheitis virus (ILTV), Fowlpox virus (FPV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), avian reovirus (ARV), reticuloendotheliosis virus (REV), avian influenza virus (AIV) and Escherichia coli. The only specimens that yielded a strong signal were ALV-J, ALV-A and ALV-B, indicating that this assay is suitable for the detection of ALV. The limit of detection of this assay was 1.25 ng/ml of rp27 protein and 10(1.79)TCID(50) units of HLJ09MDJ-1 (ALV-J). Moreover, this AC-ELISA can detect ALV in cloacal swabs of chickens experimentally infected as early as 12 days post-infection. The AC-ELISA detected the virus in the albumin and cloacal swabs of naturally infected chickens, and the results were confirmed by PCR, indicating that the AC-ELISA was a suitable method for the detection of ALV. This test is rapid and sensitive and could be convenient for epidemiological studies and eradication programs.

UI MeSH Term Description Entries
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral
D001353 Avian Leukosis A group of transmissible viral diseases of chickens and turkeys. Liver tumors are found in most forms, but tumors can be found elsewhere. Leukosis, Avian,Avian Leukoses,Leukoses, Avian
D001354 Avian Leukosis Virus The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl. Leukosis Virus, Avian,Lymphomatosis Virus, Avian,Rous-Associated Virus,Avian Leukosis Viruses,Avian Lymphomatosis Virus,Avian Lymphomatosis Viruses,Leukosis Viruses, Avian,Lymphomatosis Viruses, Avian,Rous Associated Virus,Virus, Avian Leukosis,Virus, Avian Lymphomatosis,Virus, Rous-Associated,Viruses, Avian Leukosis,Viruses, Avian Lymphomatosis
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D014730 Veterinary Medicine The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals. Medicine, Veterinary
D014773 Virology The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.

Related Publications

Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development and evaluation of an immunochromatographic strip for rapid detection of capsid protein antigen p27 of avian leukosis virus.
September 2015, Journal of virological methods,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development of an antigen-capture ELISA for the detection of equine influenza virus nucleoprotein.
July 2011, Journal of virological methods,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development of an antigen-capture ELISA for the detection of the p27-CA protein of HERV-K(HML-2).
August 2016, Journal of virological methods,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
[Correlation between TCID50 and p27 antigen of avian leukosis virus subgroup J].
November 2011, Bing du xue bao = Chinese journal of virology,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens.
February 2008, Journal of virological methods,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Application of monoclonal antibodies in the avian leukosis virus GS-antigen ELISA.
January 1985, Avian pathology : journal of the W.V.P.A,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development and Application of an Antigen Capture ELISA for the Detection of Enzootic Nasal Tumor Virus-2.
January 2025, Transboundary and emerging diseases,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development of an antigen-capture ELISA for beak and feather disease virus.
January 2018, Archives of virology,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Antigen-capture ELISA for rapid diagnosis of avian influenza virus in commercial turkey flocks.
July 1993, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Bingling Yun, and Delong Li, and Haibo Zhu, and Wen Liu, and Liting Qin, and Zaisi Liu, and Guan Wu, and Yongqiang Wang, and Xiaole Qi, and Honglei Gao, and Xiaomei Wang, and Yulong Gao
Development of a novel immuno-PCR for detection of avian leukosis virus.
October 2016, Journal of virological methods,
Copied contents to your clipboard!