Intravital two-photon microscopy of host-pathogen interactions in a mouse model of Staphylococcus aureus skin abscess formation. 2013

Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
Program of Molecular Pathogenesis, Helen L and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York City, NY 10016, USA. jan.liese@med.uni-tuebingen.de

Staphylococcus (S.) aureus is a frequent cause of severe skin infections. The ability to control the infection is largely dependent on the rapid recruitment of neutrophils (PMN). To gain more insight into the dynamics of PMN migration and host-pathogen interactions in vivo, we used intravital two-photon (2-P) microscopy to visualize S. aureus skin infections in the mouse. Reporter S. aureus strains expressing fluorescent proteins were developed, which allowed for detection of the bacteria in vivo. By employing LysM-EGFP mice to visualize PMN, we observed the rapid appearance of PMN in the extravascular space of the dermis and their directed movement towards the focus of infection, which led to the delineation of an abscess within 1 day. Moreover, tracking of transferred labelled bone-marrow neutrophils showed that PMN localization to the site of infection is dependent on the presence of G-protein-coupled receptors on the PMN, whereas Interleukin-1 receptor was required on host cells other than PMN. Furthermore, the S. aureus complement inhibitor Ecb could block PMN accumulation at thesite of infection. Our results establish that 2-P microscopy is a powerful tool to investigate the orchestration of the immune cells, S. aureus location and gene expression in vivo on a single cell level.

UI MeSH Term Description Entries
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D000038 Abscess Accumulation of purulent material in tissues, organs, or circumscribed spaces, usually associated with signs of infection. Abscesses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012867 Skin The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D013207 Staphylococcal Skin Infections Infections to the skin caused by bacteria of the genus STAPHYLOCOCCUS. Skin Diseases, Staphylococcal,Infections, Staphylococcal Skin,Skin Infections, Staphylococcal,Staphylococcal Diseases, Skin,Staphylococcal Infections, Skin,Staphylococcal Skin Diseases
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.

Related Publications

Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
February 2012, Current opinion in microbiology,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
August 2018, International journal of medical microbiology : IJMM,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
January 2009, Cell host & microbe,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
March 2024, Journal of visualized experiments : JoVE,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
April 2024, Journal of visualized experiments : JoVE,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
April 2009, Cellular microbiology,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
January 2018, Methods in molecular biology (Clifton, N.J.),
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
March 2024, eLife,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
December 2023, bioRxiv : the preprint server for biology,
Jan Liese, and Suzan H M Rooijakkers, and Jos A G van Strijp, and Richard P Novick, and Michael L Dustin
March 2014, International journal of medical microbiology : IJMM,
Copied contents to your clipboard!