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The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 microM, Bmax = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.
L R McMartin, and
R J Summers
August 1981,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
L R McMartin, and
R J Summers
January 1988,
Life sciences,
L R McMartin, and
R J Summers
July 1986,
Biochemical pharmacology,
L R McMartin, and
R J Summers
August 1996,
Kidney international,
L R McMartin, and
R J Summers
June 1983,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
L R McMartin, and
R J Summers
February 1988,
European journal of pharmacology,
L R McMartin, and
R J Summers
July 1989,
The Journal of pharmacology and experimental therapeutics,
L R McMartin, and
R J Summers
January 1988,
Pharmacology,
L R McMartin, and
R J Summers
January 1977,
Nephron,
L R McMartin, and
R J Summers
October 1984,
European journal of pharmacology,
