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The effect of low density lipoprotein (LDL) on the intracellular pH (pHi) of vascular smooth muscle cells (VSMC) was investigated using a fluorescent pH indicator, 2',7'-bis(carboxyethyl)carboxyfluorescein (BCECF). LDL and apoprotein B (apo-B), a binding protein for the LDL receptor, caused transient acidification followed by Na(+)-dependent and amiloride-sensitive alkalization of the cells due to stimulation of Na+/H+ exchanger. NH4Cl also caused intracellular alkalization, but independently of extracellular Na+. LDL, apo-B and NH4Cl all stimulated thymidine incorporation. These results indicate that the binding of LDL to its receptor stimulates Na+/H+ exchanger, resulting in alkalization of VSMC and suggest that this may function as a massage in stimulation of DNA synthesis evoked by LDL.
E Koh, and
S Morimoto, and
T Nabata, and
Y Miyashita, and
S Kitano, and
R Morita, and
T Ogihara
December 1989,
Journal of hypertension. Supplement : official journal of the International Society of Hypertension,
E Koh, and
S Morimoto, and
T Nabata, and
Y Miyashita, and
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R Morita, and
T Ogihara
January 1991,
Contributions to nephrology,
E Koh, and
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T Nabata, and
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T Ogihara
January 1994,
Gerontology,
E Koh, and
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February 1992,
Arteriosclerosis and thrombosis : a journal of vascular biology,
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February 1990,
Biochemical and biophysical research communications,
E Koh, and
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May 1995,
Biochemistry and molecular biology international,
E Koh, and
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T Ogihara
May 1994,
Biochemical Society transactions,
E Koh, and
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T Nabata, and
Y Miyashita, and
S Kitano, and
R Morita, and
T Ogihara
July 1993,
Circulation research,
E Koh, and
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T Nabata, and
Y Miyashita, and
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R Morita, and
T Ogihara
June 1990,
Hypertension (Dallas, Tex. : 1979),
E Koh, and
S Morimoto, and
T Nabata, and
Y Miyashita, and
S Kitano, and
R Morita, and
T Ogihara
January 1988,
Heart and vessels,
