Biomaterial Database

Brazilian green propolis: anti-inflammatory property by an immunomodulatory activity. 2012

Joleen Lopes Machado, and Anne Karine Martins Assunção, and Mayara Cristina Pinto da Silva, and Aramys Silva Dos Reis, and Graciomar Conceição Costa, and Diêgo de Sousa Arruda, and Bruno Alves Rocha, and Mirela Mara de Oliveira Lima Leite Vaz, and Antonio Marcus de Andrade Paes, and Rosane Nassar Meireles Guerra, and Andresa Aparecida Berretta, and Flávia Raquel Fernandes do Nascimento

Department of Pathology, Laboratory of Immunophysiology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), 65085-580 São Luis, Brazil.

The immunomodulatory and anti-inflammatory activities of green propolis extracts from Apis mellifera were investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) of Escherichia coli by intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.