-transparent.png){kind=logo}
Onchocerciasis patients treated with diethylcarbamazine often undergo a severe inflammatory response, the Mazzotti reaction. To assess the eosinophil's role in the pathogenesis of the Mazzotti reaction, we obtained serial blood, plasma, and skin biopsy specimens from 21 heavily infected patients and 3 endemic controls, both before and during therapy with diethylcarbamazine. Samples were analyzed for blood eosinophils, plasma levels of eosinophil granule major basic protein (MBP) and eosinophil-derived neurotoxin, eosinophil infiltration and eosinophil and mast cell degranulation in the skin. After the first dose of diethylcarbamazine, blood eosinophils fell from a pre-treatment level of 888 +/- 111 to 203 +/- 42 cells/mm3 at 8 h. This decrease was followed by a marked eosinophilia developing over the remaining 7 days of treatment and 14 days of follow-up. Plasma eosinophil-derived neurotoxin levels increased from 56 +/- 4 ng/ml pretreatment to a peak of 82 +/- 9 ng/ml at 8 h and returned to pretreatment levels by 48 h. Beginning at 12 h, plasma MBP levels increased from 730 +/- 74 ng/ml pretreatment to a peak of 1140 +/- 74 ng/ml after 5 days. Pretreatment skin biopsies stained for MBP by immunofluorescence showed a bright fibrillar pattern in the dermis consistent with chronic eosinophil degranulation; the MBP was localized on elastic tissue fibers. After treatment, skin biopsy specimens showed both the pretreatment fibrillar MBP staining pattern as well as focal eosinophil degranulation. Deposition of MBP around microfilariae in the papillary dermis was visible as early as 1.5 h. The lowest blood eosinophil levels and peak plasma eosinophil-derived neurotoxin levels coincided with the infiltration and degranulation of eosinophils in the skin. Mast cell degranulation in the skin was maximal by the first posttreatment biopsy (1.5 h) coincident with the beginning of eosinophil degranulation. Although the pathogenesis of the Mazzotti reaction is clearly complex, our results indicate that eosinophil degranulation is characteristic of the response and that it occurs with a time course suggestive of a role for the eosinophil in determining the clinical and pathologic manifestations of the reaction.
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
December 1982,
Leprosy review,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
March 1988,
Acta tropica,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
June 1987,
Lancet (London, England),
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
June 1982,
Annals of tropical medicine and parasitology,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
October 1982,
Annals of tropical medicine and parasitology,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
June 1982,
Annals of tropical medicine and parasitology,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
September 1993,
Investigative ophthalmology & visual science,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
August 1989,
Archives of dermatology,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
January 1987,
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie,
S J Ackerman, and
G M Kephart, and
H Francis, and
K Awadzi, and
G J Gleich, and
E A Ottesen
January 2001,
International archives of allergy and immunology,
