BIOMAT Database Logo BIOMAT Database Logo

The effect of currently available contact lens disinfection systems on Acanthamoeba castellanii and Acanthamoeba polyphaga. 1990

R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas.

Contact lens disinfection systems were evaluated for their effectiveness in killing Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites and cysts. Amoebae were inoculated into commercially available contact lens cleaning and soaking solutions. At intervals varying from 30 minutes to 24 hours, solutions were filtered. The filters were removed and cultured for Acanthamoeba organisms. Striking differences were observed in the abilities of the different disinfecting solutions to kill the organisms. Solutions containing chlorhexidine were effective at very short exposure times. Solutions containing benzalkonium chloride required slightly longer exposure times but were faster than solutions containing only thimerosal. Solutions containing sorbate, polyaminopropyl biguanide, or polyquaternium-1 were not effective at killing Acanthamoeba organisms in the time allotted for the experiment. Solutions containing hydrogen peroxide were quite effective if the agent was not prematurely catalyzed. A. polyphaga generally required longer exposure to disinfectants than did A. castellanii for complete inhibition to occur.

UI MeSH Term Description Entries
D009883 Ophthalmic Solutions Sterile solutions that are intended for instillation into the eye. It does not include solutions for cleaning eyeglasses or CONTACT LENS SOLUTIONS. Eye Drop,Eyedrop,Eyedrops,Ophthalmic Solution,Eye Drops,Drop, Eye,Drops, Eye,Solution, Ophthalmic,Solutions, Ophthalmic
D003261 Contact Lenses Lenses designed to be worn on the front surface of the eyeball. (UMDNS, 1999) Lenses, Contact,Contact Lens,Lens, Contact
D004202 Disinfectants Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed) Biocide,Disinfectant,Biocides
D004866 Equipment Contamination The presence of an infectious agent on instruments, prostheses, or other inanimate articles. Contamination, Equipment,Contaminations, Equipment,Equipment Contaminations
D000048 Acanthamoeba A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract. Acanthamoebas
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015169 Colony Count, Microbial Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing. Agar Dilution Count,Colony-Forming Units Assay, Microbial,Fungal Count,Pour Plate Count,Spore Count,Spread Plate Count,Streak Plate Count,Colony Forming Units Assay, Microbial,Colony Forming Units Assays, Microbial,Agar Dilution Counts,Colony Counts, Microbial,Count, Agar Dilution,Count, Fungal,Count, Microbial Colony,Count, Pour Plate,Count, Spore,Count, Spread Plate,Count, Streak Plate,Counts, Agar Dilution,Counts, Fungal,Counts, Microbial Colony,Counts, Pour Plate,Counts, Spore,Counts, Spread Plate,Counts, Streak Plate,Dilution Count, Agar,Dilution Counts, Agar,Fungal Counts,Microbial Colony Count,Microbial Colony Counts,Pour Plate Counts,Spore Counts,Spread Plate Counts,Streak Plate Counts

Related Publications

R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Comparison of hydrogen peroxide contact lens disinfection systems and solutions against Acanthamoeba polyphaga.
July 2001, Antimicrobial agents and chemotherapy,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Potential resistant morphotypes of Acanthamoeba castellanii expressed in multipurpose contact lens disinfection systems.
November 2012, Eye & contact lens,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Acanthamoeba culbertsoni and contact lens disinfection systems.
October 1989, Optometry and vision science : official publication of the American Academy of Optometry,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii.
January 2006, Canadian journal of microbiology,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Susceptibility of Acanthamoeba to soft contact lens disinfection systems.
April 1986, Investigative ophthalmology & visual science,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Effectivity of contact lens disinfection systems against Acanthamoeba culbertsoni.
February 1991, Optometry and vision science : official publication of the American Academy of Optometry,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
The effectiveness of soft contact lens disinfection systems against Acanthamoeba on the lens surface.
March 1996, Journal of the American Optometric Association,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Susceptibility of Acanthamoeba castellanii to contact lens disinfecting solutions.
July 1995, Antimicrobial agents and chemotherapy,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Inefficacy of marketed contact lens disinfection solutions against keratitis-causing Acanthamoeba castellanii belonging to the T4 genotype.
June 2014, Experimental parasitology,
R E Silvany, and J M Dougherty, and J P McCulley, and T S Wood, and R W Bowman, and M B Moore
Acanthamoeba and contact lens disinfection: should chlorine be discontinued?
February 1993, The British journal of ophthalmology,
Copied contents to your clipboard!