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We have examined the use of pulsed-field gel electrophoresis (PFGE) to measure DNA double-strand breaks induced in CHO cells by ionizing radiation. The PFGE assay provides a simple method for the measurement of DNA double-strand breaks for doses as low as 3-4 Gy ionizing radiation, and appears applicable for the measurement of damage produced by any agent producing double-strand breaks. The conditions of transverse alternating field electrophoresis determined both the sensitivity of the assay and the ability to resolve DNA fragments with different sizes. For example, with 0.8% agarose and a 1-min pulse time at 250 V for 18 h of electrophoresis, 0.39% of the DNA per gray migrated into the gel, and only molecules less than 1500 kb could be resolved. With 0.56% agarose and a 60-min pulse time at 40 V for 6 days of electrophoresis, 0.55-0.90% of the DNA per gray migrated into the gel, and molecules between 1500 and 7000 kb could be resolved.
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C A Waldren
January 2017,
Genes to cells : devoted to molecular & cellular mechanisms,
D D Ager, and
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W Harvey, and
R T Johnson, and
C A Waldren
January 1997,
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D D Ager, and
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C A Waldren
January 2019,
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D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
March 1993,
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D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 1994,
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group,
D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 2002,
Methods in molecular biology (Clifton, N.J.),
D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 2020,
Methods in molecular biology (Clifton, N.J.),
D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 2020,
Methods in molecular biology (Clifton, N.J.),
D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 2020,
Methods in molecular biology (Clifton, N.J.),
D D Ager, and
W C Dewey, and
K Gardiner, and
W Harvey, and
R T Johnson, and
C A Waldren
January 2019,
Methods in molecular biology (Clifton, N.J.),
