BIOMAT Database Logo BIOMAT Database Logo

Purification and amino acid sequence of vasoactive intestinal peptide, peptide histidine isoleucinamide (1-27) and secretin from the small intestine of guinea pig. 1990

L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.

The neuropeptides vasoactive intestinal peptide (VIP) and peptide histidine isoleucinamide (1-27) (PHI) and the hormone secretin were purified from the small intestine of guinea pig, being detected by radioimmunoassay and radioreceptor assay throughout six to seven chromatographic steps. After elution on a reverse-phase C18 column, the three peptides were separated on a Fractogel column. After cation-exchange chromatography of each peptide on Mono S, the final steps were performed using a reverse-phase RP8-e column. Guinea pig PHI differed from porcine PHI in having Tyr and Arg residues instead of Phe and Lys in, respectively, position 10 and 20. We confirmed the original sequence of guinea pig VIP previously documented (with Leu5, Thr9, Met19 and Val26). We also established the similarity of the primary structure of guinea pig secretin with that of porcine and bovine.

UI MeSH Term Description Entries
D007421 Intestine, Small The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM. Small Intestine,Intestines, Small,Small Intestines
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010451 Peptide PHI A 27-amino acid peptide with histidine at the N-terminal and isoleucine amide at the C-terminal. The exact amino acid composition of the peptide is species dependent. The peptide is secreted in the intestine, but is found in the nervous system, many organs, and in the majority of peripheral tissues. It has a wide range of biological actions, affecting the cardiovascular, gastrointestinal, respiratory, and central nervous systems. Neuropeptide PHI 27,PHI Peptide,Peptide Histidine-Isoleucine,Peptide PHM,Human PHI,PHM Neuropeptide,PHM-27,Peptide HI,Peptide Histidine Methionine,Peptide PHI-27,Peptide-Histidine-Isoleucinamide,Porcine Intestinal Heptacosapeptide,Pro-Vasoactive Intestinal Peptide,Heptacosapeptide, Porcine Intestinal,Histidine Methionine, Peptide,Histidine-Isoleucine, Peptide,Intestinal Heptacosapeptide, Porcine,Intestinal Peptide, Pro-Vasoactive,Methionine, Peptide Histidine,Neuropeptide, PHM,PHI, Human,PHM, Peptide,Peptide Histidine Isoleucinamide,Peptide Histidine Isoleucine,Peptide PHI 27,Peptide, PHI,Peptide, Pro-Vasoactive Intestinal,Pro Vasoactive Intestinal Peptide
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Purification and amino acid sequence of vasoactive intestinal peptide, peptide histidine isoleucinamide and secretin from the ovine small intestine.
February 1991, Regulatory peptides,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Peptide histidine isoleucinamide (PHI)-(1-27)-Gly as a new major form of PHI in the rat small intestine.
September 1989, Endocrinology,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Receptors for vasoactive intestinal peptide and secretin on guinea pig pancreatic acini.
January 1987, Peptides,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Actions of vasoactive intestinal peptide and secretin on chief cells prepared from guinea pig stomach.
July 1986, The American journal of physiology,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Structure of the vasoactive intestinal octacosapeptide from chicken intestine. The amino acid sequence.
December 1975, FEBS letters,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Ontogeny of immunoreactive cholecystokinin, vasoactive intestinal peptide, and secretin in guinea pig brain and gut.
March 1986, Endocrinology,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Isolation, amino acid composition and terminal amino acid residues of the vasoactive actacosapeptide from chicken intestine. Partial purification of chicken secretin.
October 1974, FEBS letters,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
The amino acid sequence of guinea pig C-peptide.
March 1974, Nature,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
Purification and amino acid sequence of motilin from cat small intestine.
November 1993, Regulatory peptides,
L Buscail, and A Cauvin, and P Gourlet, and D Gossen, and P De Neef, and J Rathé, and P Robberecht, and M C Vandermeers-Piret, and A Vandermeers, and J Christophe
[D-Phe4]peptide histidine-isoleucinamide ([D-Phe4]PHI), a highly selective vasoactive-intestinal-peptide (VIP) agonist, discriminates VIP-preferring from secretin-preferring receptors in rat pancreatic membranes.
June 1987, European journal of biochemistry,
Copied contents to your clipboard!