BIOMAT Database Logo BIOMAT Database Logo

Alterations in membrane fluidity during keratinocyte differentiation measured by fluorescence polarization. 1990

H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Department of Dermatology, Kurume University School of Medicine, Japan.

The epidermis shows a distinctive pattern of differentiation wherein keratinocytes proliferate in the basal cell layer and mature into spinous and granular cells. Using a discontinuous density-gradient centrifugation method, guinea-pig keratinocytes were separated into high (HDF), intermediate (IDF), and low (LDF) density fractions. Morphological and flow cytometrical observations demonstrated that HDF, IDF, and LDF were basal, spinous, and granular cell-rich fractions, respectively. Membrane fluidity of the fractionated keratinocytes was measured by diphenylhexatriene fluorescence polarization. Polarization (p)-value of keratinocytes was negatively correlated with temperature. At each temperature, HDF cells showed a lower p-value than IDF or HDF cells except at 40 degrees C. Since a low p-value indicates a high degree of Brownian motion, membrane fluidity is higher in basal cells and lower in spinous and granular cells. Our results indicate that membrane fluidity of guinea-pig keratinocytes decreases during their maturation.

UI MeSH Term Description Entries
D008297 Male Males
D008560 Membrane Fluidity The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature. Bilayer Fluidity,Bilayer Fluidities,Fluidities, Bilayer,Fluidities, Membrane,Fluidity, Bilayer,Fluidity, Membrane,Membrane Fluidities
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002458 Cell Fractionation Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS. Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D002499 Centrifugation, Density Gradient Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D005260 Female Females
D005454 Fluorescence Polarization Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. Anisotropy, Fluorescence,Fluorescence Anisotropy,Polarization, Fluorescence,Anisotropies, Fluorescence,Fluorescence Anisotropies,Fluorescence Polarizations,Polarizations, Fluorescence
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015603 Keratinocytes Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell. Keratinocyte

Related Publications

H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Membrane fluidity measured by fluorescence polarization using an EPICS V cell sorter.
January 1987, Cytometry,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
[Fluorescence polarization used to derive cell membrane fluidity during photodynamic therapy].
November 2005, Guang pu xue yu guang pu fen xi = Guang pu,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Membrane fluidity of Toxoplasma gondii: a fluorescence polarization study.
January 1988, Biology of the cell,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity.
January 2021, Methods in molecular biology (Clifton, N.J.),
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Cheek cell membrane fluidity measured by fluorescence recovery after photobleaching and steady-state fluorescence anisotropy.
November 1994, The Journal of membrane biology,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Cellular membrane fluidity measurement by fluorescence polarization in indomethacin-induced gastric cellular injury in vitro.
December 2007, Journal of gastroenterology,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Fluorescence polarization in studies of bacterial cytoplasmic membrane fluidity under environmental stress.
January 2007, Progress in biophysics and molecular biology,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Plasma membrane fluidity studies by fluorescence polarization in rat mast cells stimulated by compound 48/80.
April 1985, Agents and actions,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
[Effect of 3,4-dihydroxyacetophenone on the membrane fluidity of platelets by fluorescence polarization analysis].
March 1986, Zhongguo yao li xue bao = Acta pharmacologica Sinica,
H Hachisuka, and H Nomura, and O Mori, and S Nakano, and K Okubo, and M Kusuhara, and M Karashima, and E Tanikawa, and M Higuchi, and Y Sasai
Alterations of membrane fluidity by toxic injuries.
January 1992, Renal failure,
Copied contents to your clipboard!