BIOMAT Database Logo BIOMAT Database Logo

BnSP-7 toxin, a basic phospholipase A2 from Bothrops pauloensis snake venom, interferes with proliferation, ultrastructure and infectivity of Leishmania (Leishmania) amazonensis. 2013

Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.

This paper reports the effects of BnSP-7 toxin, a catalytically inactive phospholipase A2 from Bothrops pauloensis snake venom, on Leishmania (Leishmania) amazonensis. BnSP-7 presented activity against promastigote parasite forms both in the MTT assay, with IC50 of 58.7 μg mL(-1) of toxin, and a growth curve, inhibiting parasite proliferation 60-70% at concentrations of 50-200 μg mL(-1) of toxin 96 h after treatment. Also, the toxin presented effects on amastigotes, reducing parasite viability by 50% at 28.1 μg mL(-1) and delaying the amastigote-promastigote differentiation process. Ultrastructural studies showed that BnSP-7 caused severe morphological changes in promastigotes such as mitochondrial swelling, nuclear alteration, vacuolization, acidocalcisomes, multiflagellar aspects and a blebbing effect in the plasma membrane. Finally, BnSP-7 interfered with the infective capacity of promastigotes in murine peritoneal macrophages, causing statistically significant infectivity-index reductions (P < 0.05) of 20-35%. These data suggest that the BnSP-7 toxin is an important tool for the discovery of new parasite targets that can be exploited to develop new drugs for treating leishmaniasis.

UI MeSH Term Description Entries
D007891 Leishmania A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi. Leishmania (Leishmania),Leishmania (Viannia),Leishmania leishmania,Leishmania viannia,Leishmania leishmanias,Leishmania viannias,Leishmanias,Leishmanias (Leishmania),Leishmanias (Viannia),leishmanias, Leishmania,viannias, Leishmania
D007896 Leishmaniasis A disease caused by any of a number of species of protozoa in the genus LEISHMANIA. There are four major clinical types of this infection: cutaneous (Old and New World) (LEISHMANIASIS, CUTANEOUS), diffuse cutaneous (LEISHMANIASIS, DIFFUSE CUTANEOUS), mucocutaneous (LEISHMANIASIS, MUCOCUTANEOUS), and visceral (LEISHMANIASIS, VISCERAL). Leishmania Infection,Infection, Leishmania,Infections, Leishmania,Leishmania Infections,Leishmaniases
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003435 Crotalid Venoms Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized. Bothrops Venom,Crotalidae Venoms,Pit Viper Venoms,Rattlesnake Venoms,Crotactin,Crotalid Venom,Crotalin,Crotaline Snake Venom,Crotalotoxin,Crotamin,Pit Viper Venom,Rattlesnake Venom,Snake Venom, Crotaline,Venom, Bothrops,Venom, Crotalid,Venom, Crotaline Snake,Venom, Pit Viper,Venom, Rattlesnake,Venoms, Crotalid,Venoms, Crotalidae,Venoms, Pit Viper,Venoms, Rattlesnake,Viper Venom, Pit
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017737 Macrophages, Peritoneal Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum. Peritoneal Macrophages,Macrophage, Peritoneal,Peritoneal Macrophage
D046529 Microscopy, Electron, Transmission Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen. Electron Diffraction Microscopy,Electron Microscopy, Transmission,Microscopy, Electron Diffraction,Transmission Electron Microscopy,Diffraction Microscopy, Electron,Microscopy, Transmission Electron
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach.
April 2021, Toxicology in vitro : an international journal published in association with BIBRA,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Anti-parasitic effect on Toxoplasma gondii induced by BnSP-7, a Lys49-phospholipase A2 homologue from Bothrops pauloensis venom.
September 2016, Toxicon : official journal of the International Society on Toxinology,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom.
June 2000, Archives of biochemistry and biophysics,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
The Anthelmintic Effect on Strongyloides venezuelensis Induced by BnSP- 6, a Lys49-phospholipase A2 Homologue from Bothrops pauloensis Venom.
January 2019, Current topics in medicinal chemistry,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells.
October 2018, International journal of biological macromolecules,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Insights of local tissue damage and regeneration induced by BnSP-7, a myotoxin isolated from Bothrops (neuwiedi) pauloensis snake venom.
April 2009, Toxicon : official journal of the International Society on Toxinology,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Isolation and enzymatic characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom.
April 2001, Journal of protein chemistry,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
BmajPLA2-II, a basic Lys49-phospholipase A2 homologue from Bothrops marajoensis snake venom with parasiticidal potential.
September 2017, International journal of biological macromolecules,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Bactericidal and neurotoxic activities of two myotoxic phospholipases A2 from Bothrops neuwiedi pauloensis snake venom.
September 2004, Toxicon : official journal of the International Society on Toxinology,
Débora C O Nunes, and Márcia M N R Figueira, and Daiana S Lopes, and Dayane L Naves De Souza, and Luiz Fernando M Izidoro, and Eloísa A V Ferro, and Maria A Souza, and Renata S Rodrigues, and Veridiana M Rodrigues, and Kelly A G Yoneyama
Antiparasitic effects induced by polyclonal IgY antibodies anti-phospholipase A2 from Bothrops pauloensis venom.
June 2018, International journal of biological macromolecules,
Copied contents to your clipboard!