BIOMAT Database Logo BIOMAT Database Logo

Indole-3-acetic acid biosynthetic pathway and aromatic amino acid aminotransferase activities in Pantoea dispersa strain GPK. 2013

G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India.

This investigation deals with the production of IAA by a bacterial isolate Pantoea dispersa strain GPK (PDG) identified by 16S rRNA gene sequence analysis. HPLC and Mass spectral analysis of metabolites from bacterial spent medium revealed that, IAA production by PDG is Trp-dependent and follows indole-3-pyruvic acid (IPyA) pathway. Substrate specificity study of aromatic amino acid aminotransferase (AAT) showed high activities, only when tryptophan (Trp) and α-ketoglutarate (α-kg) were used as substrates. AAT is highly specific for Trp and α-kg as amino group donor and acceptor, respectively. The effect of exogenous IAA on bacterial growth was established. Low concentration of exogenous IAA induced the growth, whereas high concentration decreased the growth of bacterium. PDG treatment significantly increased the root length, shoot length and dry mass of the chickpea and pigeon pea plants.

UI MeSH Term Description Entries
D007210 Indoleacetic Acids Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed) Auxin,Auxins,Indolylacetic Acids,Acids, Indoleacetic,Acids, Indolylacetic
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D007656 Ketoglutaric Acids A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442) Oxoglutarates,2-Ketoglutarate,2-Ketoglutaric Acid,2-Oxoglutarate,2-Oxoglutaric Acid,Calcium Ketoglutarate,Calcium alpha-Ketoglutarate,Ketoglutaric Acid,Oxogluric Acid,alpha-Ketoglutarate,alpha-Ketoglutaric Acid,alpha-Ketoglutaric Acid, Calcium Salt (2:1),alpha-Ketoglutaric Acid, Diammonium Salt,alpha-Ketoglutaric Acid, Dipotassium Salt,alpha-Ketoglutaric Acid, Disodium Salt,alpha-Ketoglutaric Acid, Monopotassium Salt,alpha-Ketoglutaric Acid, Monosodium Salt,alpha-Ketoglutaric Acid, Potassium Salt,alpha-Ketoglutaric Acid, Sodium Salt,alpha-Oxoglutarate,2 Ketoglutarate,2 Ketoglutaric Acid,2 Oxoglutarate,2 Oxoglutaric Acid,Calcium alpha Ketoglutarate,alpha Ketoglutarate,alpha Ketoglutaric Acid,alpha Ketoglutaric Acid, Diammonium Salt,alpha Ketoglutaric Acid, Dipotassium Salt,alpha Ketoglutaric Acid, Disodium Salt,alpha Ketoglutaric Acid, Monopotassium Salt,alpha Ketoglutaric Acid, Monosodium Salt,alpha Ketoglutaric Acid, Potassium Salt,alpha Ketoglutaric Acid, Sodium Salt,alpha Oxoglutarate,alpha-Ketoglutarate, Calcium
D000637 Transaminases A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1. Aminotransferase,Aminotransferases,Transaminase
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014364 Tryptophan An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals. Ardeydorm,Ardeytropin,L-Tryptophan,L-Tryptophan-ratiopharm,Levotryptophan,Lyphan,Naturruhe,Optimax,PMS-Tryptophan,Trofan,Tryptacin,Tryptan,Tryptophan Metabolism Alterations,ratio-Tryptophan,L Tryptophan,L Tryptophan ratiopharm,PMS Tryptophan,ratio Tryptophan
D053898 Biosynthetic Pathways Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds. Biosynthetic Pathway,Pathway, Biosynthetic,Pathways, Biosynthetic
D020636 Pantoea A genus of gram-negative, facultatively anaerobic, straight rods which are motile by peritrichous flagella. Most strains produce a yellow pigment. This organism is isolated from plant surfaces, seeds, soil, and water, as well as from animals and human wounds, blood, and urine. (From Bergey's Manual of Determinative Bacteriology, 9th ed) Pantoea agglomerans
D029863 Cicer A plant genus of the family FABACEAE known for the edible beans. Chickpea,Garbanzo,Cicer arietinum,Chickpeas,Cicer arietinums,Cicers,Garbanzos,arietinum, Cicer
D036561 Cajanus A plant genus of the family FABACEAE that is used for food in NIGERIA. Pea Plant, Pigeon,Pigeonpea Plant,Cajanus cajan,Cajanus cajans,Pea Plants, Pigeon,Pigeon Pea Plant,Pigeon Pea Plants,Pigeonpea Plants,Plant, Pigeon Pea,Plant, Pigeonpea,Plants, Pigeon Pea,Plants, Pigeonpea,cajan, Cajanus

Related Publications

G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Aromatic amino acid aminotransferase activity and indole-3-acetic acid production by associative nitrogen-fixing bacteria.
April 2004, FEMS microbiology letters,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Indole-3-acetic acid biosynthesis in Fusarium delphinoides strain GPK, a causal agent of Wilt in Chickpea.
February 2013, Applied biochemistry and biotechnology,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis.
January 2019, Mycobiology,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM.
May 2011, Journal of applied microbiology,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand.
January 2020, PloS one,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Biosynthetic Pathways and Functions of Indole-3-Acetic Acid in Microorganisms.
August 2023, Microorganisms,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid.
November 1993, Proceedings of the National Academy of Sciences of the United States of America,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana.
November 2000, The Plant journal : for cell and molecular biology,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Regulation of indole-3-acetic Acid biosynthetic pathways in carrot cell cultures.
November 1992, Plant physiology,
G B Kulkarni, and A S Nayak, and S S Sajjan, and A Oblesha, and T B Karegoudar
Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum.
July 2016, Archives of microbiology,
Copied contents to your clipboard!