BIOMAT Database Logo BIOMAT Database Logo

Low-grade stromal sarcoma: DNA flow cytometric analysis and estrogen progesterone receptor data. 1990

C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Department of Obstetrics and Gynecology, Hospital of University of Pennsylvania, Philadelphia.

DNA flow cytometry (FCM) data and estrogen receptor (ER) and progesterone receptor (PR) status were studied in three cases of low-grade stromal sarcoma (LGSS). One case was a primary presentation and the remaining two were recurrent tumors. DNA FCM showed a DNA index (DI) equal to 1.00, consistent with a diploid cell population, for four of the six specimens studied. The other two showed near-diploid populations. Proliferation indices (PI) were low in two of the patients' tumors (8.0 and 12.7%). These findings are consistent with the clinical history of LGSS and its propensity for indolent growth, long intervals between recurrences, and generally favorable prognosis. In case 2, a patient with several recurrences, the PI was increased to 20.3% in a specimen from the first recurrence. She subsequently recurred within 1 year with a more aggressive tumor, characterized by a mitotic index of greater than 10 mitoses/10 high-power fields (HPF), absence of ER and PR, and an aneuploid population (DI = 1.19). Receptor data, obtained by dextran-coated charcoal assay, showed that all tumors except the aggressive recurrence in case 2 had high ER (average 316 fmole/mg protein) and high PR (average 753 fmole/mg protein) levels. These ER and PR data are similar to the two other reports in the literature and the usual clinical response to progestational therapy was demonstrated. Further studies will help define the possible role of FCM and ER and PR determinations in patients with LGSS. These preliminary data suggest that they may be of prognostic significance.

UI MeSH Term Description Entries
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009364 Neoplasm Recurrence, Local The local recurrence of a neoplasm following treatment. It arises from microscopic cells of the original neoplasm that have escaped therapeutic intervention and later become clinically visible at the original site. Local Neoplasm Recurrence,Local Neoplasm Recurrences,Locoregional Neoplasm Recurrence,Neoplasm Recurrence, Locoregional,Neoplasm Recurrences, Local,Recurrence, Local Neoplasm,Recurrence, Locoregional Neoplasm,Recurrences, Local Neoplasm,Locoregional Neoplasm Recurrences,Neoplasm Recurrences, Locoregional,Recurrences, Locoregional Neoplasm
D011960 Receptors, Estrogen Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important. Estrogen Receptor,Estrogen Receptors,Estrogen Nuclear Receptor,Estrogen Receptor Type I,Estrogen Receptor Type II,Estrogen Receptors Type I,Estrogen Receptors Type II,Receptor, Estrogen Nuclear,Receptors, Estrogen, Type I,Receptors, Estrogen, Type II,Nuclear Receptor, Estrogen,Receptor, Estrogen
D011980 Receptors, Progesterone Specific proteins found in or on cells of progesterone target tissues that specifically combine with progesterone. The cytosol progesterone-receptor complex then associates with the nucleic acids to initiate protein synthesis. There are two kinds of progesterone receptors, A and B. Both are induced by estrogen and have short half-lives. Progesterone Receptors,Progestin Receptor,Progestin Receptors,Receptor, Progesterone,Receptors, Progestin,Progesterone Receptor,Receptor, Progestin
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004715 Endometriosis A condition in which functional endometrial tissue is present outside the UTERUS. It is often confined to the PELVIS involving the OVARY, the ligaments, cul-de-sac, and the uterovesical peritoneum. Endometrioma,Endometriomas,Endometrioses
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults

Related Publications

C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Extra-uterine endometrial stromal sarcoma with DNA flow cytometric analysis.
January 1992, European journal of obstetrics, gynecology, and reproductive biology,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Flow cytometric analysis of endometrial stromal sarcoma.
February 1992, American journal of clinical pathology,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Cytologic diagnosis of low grade endometrial stromal sarcoma by staining for estrogen and progesterone receptors.
January 2002, Acta cytologica,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Expression of progesterone receptor A and B isoforms in low-grade endometrial stromal sarcoma.
April 2004, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Flow cytometric analysis of estrogen, progesterone receptor expression and DNA content in formalin-fixed, paraffin-embedded human breast tumors.
April 1999, Cytometry,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Clinicopathologic correlation of DNA flow cytometric content analysis (DFCA), surgical staging, and estrogen/progesterone receptor status in endometrial adenocarcinoma.
April 1996, American journal of clinical oncology,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Flow and image cytometric DNA analysis in Ewing's sarcoma.
January 1992, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Polymorphous low-grade adenocarcinoma: flow cytometric, p53, and PCNA analysis.
October 1997, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Transition from low-grade endometrial stromal sarcoma to high-grade endometrial stromal sarcoma.
July 2010, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists,
C J Dunton, and M L Kelsten, and S E Brooks, and M J Viglione, and J A Carlson, and J J Mikuta
Epithelioid sarcoma: an electron-microscopic, immunohistochemical and DNA flow cytometric analysis.
January 1992, Virchows Archiv. A, Pathological anatomy and histopathology,
Copied contents to your clipboard!