Biomaterial Database

Differences in adhesion of urothelial cells to a panel of substrates are characteristic for established human urothelial cell lines differing in their transformation grade. 1990

K Ostrowski, and J Kieler, and I Balslev

Department of Histology, Medical Academy in Warsaw, Poland.

Eight human urothelial cell lines established and propagated in the Fibiger Institute, differing in their transformation grade as described by Christensen et al were brought into suspension and contacted with a panel of seven substrates, ie. Con. A, Fibronectin, WGA, Collagen IV, Laminin, PNA and artificial basal membrane. Cell adhesion was measured on big cell populations with the use of flow cytophotometry, measured samples contained nonadhering cells and fluorescent beads, added as an internal standard. It was found that the cell lines differed from each other by the kinetics of cell adhesion to the chosen panel. A higher percentage of cells belonging to established cell lines adhered to PNA, WGA or Con A coated surfaces than to laminin, fibronectin or collagen IV. The difference in adherence of cells to fibronectin, laminin or collagen IV coated surfaces makes differentiation between the single cell lines possible. Discriminant analysis of the total patterns of adhesion of all cell lines was used for comparison. The effect of trypsinization on the structure of the cell surface, accompanied by possible changes in adherence, was evaluated by comparison of the immediate results with those obtained with cells after 3 hours of recovery at 37 degrees C. It was found that all cell lines showed a better adherence to laminin after recovery. This is consistent with the published data on the high sensitivity of laminin receptors to trypsinization. On the other hand, the receptors for PNA, WGA and Con A were less expressed after the recovery period, as evidenced by a decreased adherence of the cells to these three substrates. The effect of recovery of cells on the amount of their receptors for fibronectin and collagen IV was different in different cell lines, but the direction of change for this pair of receptors was identical in a given cell line. In some instances the recovery period had no effect whatsoever on cell adherence. The different pattern of adhesion of cells belonging to established cell lines to the chosen panel of substrates can be used as a parameter for characterization of single cell lines, but it does not correlate with their grade of transformation.

UI	MeSH Term	Description	Entries
D001743	Urinary Bladder	A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.	Bladder,Bladder Detrusor Muscle,Detrusor Urinae,Bladder Detrusor Muscles,Bladder, Urinary,Detrusor Muscle, Bladder,Detrusor Muscles, Bladder
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002461	Cell Line, Transformed	Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.	Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005434	Flow Cytometry	Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.	Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014513	Ureter	One of a pair of thick-walled tubes that transports urine from the KIDNEY PELVIS to the URINARY BLADDER.	Ureters