BIOMAT Database Logo BIOMAT Database Logo

Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by polymerase chain reaction. 1990

T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.

The presence of human papillomavirus genomes-16 and -6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. Human papillomavirus DNAs extracted from paraffin-embedded tumor tissues were used for polymerase chain reaction with amplification of the E6 region of human papillomavirus genome-16 and the E1 region of human papillomavirus genome-6b. Human papillomavirus genome-16 sequences were positively amplified in six (17.6%) metastatic tumors; -6b sequence was positively amplified in one (2.9%) metastatic tumor. Laryngeal carcinomas of glottic origin showed high human papillomavirus genome-16 DNA-positive rates (4 of 9 cases, 44.4%) compared to those of other sites. These results suggest that human papillomavirus genome-16 infection might be closely associated with the development of some laryngeal squamous cell carcinomas of glottic origin similar to uterine cervical carcino-genesis.

UI MeSH Term Description Entries
D007822 Laryngeal Neoplasms Cancers or tumors of the LARYNX or any of its parts: the GLOTTIS; EPIGLOTTIS; LARYNGEAL CARTILAGES; LARYNGEAL MUSCLES; and VOCAL CORDS. Cancer of Larynx,Laryngeal Cancer,Larynx Neoplasms,Cancer of the Larynx,Larynx Cancer,Neoplasms, Laryngeal,Cancer, Laryngeal,Cancer, Larynx,Cancers, Laryngeal,Cancers, Larynx,Laryngeal Cancers,Laryngeal Neoplasm,Larynx Cancers,Larynx Neoplasm,Neoplasm, Laryngeal,Neoplasm, Larynx,Neoplasms, Larynx
D008207 Lymphatic Metastasis Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system. Lymph Node Metastasis,Lymph Node Metastases,Lymphatic Metastases,Metastasis, Lymph Node
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002294 Carcinoma, Squamous Cell A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed) Carcinoma, Epidermoid,Carcinoma, Planocellular,Carcinoma, Squamous,Squamous Cell Carcinoma,Carcinomas, Epidermoid,Carcinomas, Planocellular,Carcinomas, Squamous,Carcinomas, Squamous Cell,Epidermoid Carcinoma,Epidermoid Carcinomas,Planocellular Carcinoma,Planocellular Carcinomas,Squamous Carcinoma,Squamous Carcinomas,Squamous Cell Carcinomas
D005260 Female Females
D005784 Gene Amplification A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication. Amplification, Gene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015342 DNA Probes Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal
D015344 DNA Probes, HPV DNA probes specific for the identification of human papilloma virus. HPV DNA Probes,Human Papilloma Virus DNA Probes,Papilloma Virus DNA Probes,Probes, HPV DNA

Related Publications

T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of human papillomavirus DNA in laryngeal squamous cell carcinoma by polymerase chain reaction.
May 1996, European journal of cancer (Oxford, England : 1990),
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
[Detection of human papillomavirus DNA in squamous cell carcinomas of the lung by multiple polymerase chain reaction].
October 1993, Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
General primer-mediated polymerase chain reaction for simultaneous detection and typing of human papillomavirus DNA in laryngeal squamous cell carcinomas.
February 1995, Clinical otolaryngology and allied sciences,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of human papillomavirus in squamous cell carcinomas of the lung by polymerase chain reaction.
February 1998, American journal of respiratory cell and molecular biology,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of human papillomavirus DNA in colorectal carcinomas by polymerase chain reaction.
July 1995, Gut,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of human papillomavirus (HPV) DNA in oral squamous cell carcinomas by in situ hybridization and polymerase chain reaction.
January 1990, Archives of dermatological research,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of human papillomavirus DNA in esophageal squamous cell carcinomas by the polymerase chain reaction using general consensus primers.
September 1994, Human pathology,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction.
May 1995, Cancer,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction.
June 1994, Cancer,
T Hoshikawa, and T Nakajima, and H Uhara, and M Gotoh, and Y Shimosato, and K Tsutsumi, and I Ono, and S Ebihara
Detection of type 16 human papillomavirus DNA in formalin-fixed invasive squamous cells from laryngeal cancers by polymerase chain reaction.
January 1998, Memorias do Instituto Oswaldo Cruz,
Copied contents to your clipboard!