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The relative proportion of the two putative heavy chains of smooth muscle myosin (MHC1 and MHC2) was determined in the caudal and femoral arteries of spontaneously hypertensive rats (SHR) and normotensive (WKY) rats at 16 weeks of age. The heavy chain polypeptides with Mr 204,000 and 200,000 were resolved electrophoretically under denaturing conditions in porous polyacrylamide gels. Both proteins reacted strongly with a monoclonal antibody (2C4) to smooth muscle MHC. In caudal arteries the ratio of MHC1/MHC2 was 3.1:1 in SHR rats compared with 1.8:1 in WKY rats (p less than 0.005) and similarly in femoral arteries, 2.8:1 vs 1.5:1 (p less than 0.001). In the portal vein there was no significant difference, 1.7:1 vs 1.5:1. The possibility that the higher MHC ratio in the SHR is the genetically mediated defect in arterial smooth muscle cells leading to the hypertension is discussed as an alternative to the elevated systemic blood pressure causing the altered MHC ratio.
M P Sparrow, and
H W Mitchell, and
A W Everett
May 1997,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
M P Sparrow, and
H W Mitchell, and
A W Everett
January 1989,
European journal of biochemistry,
M P Sparrow, and
H W Mitchell, and
A W Everett
January 1991,
Advances in experimental medicine and biology,
M P Sparrow, and
H W Mitchell, and
A W Everett
July 1995,
Clinical science (London, England : 1979),
M P Sparrow, and
H W Mitchell, and
A W Everett
September 2000,
Microscopy research and technique,
M P Sparrow, and
H W Mitchell, and
A W Everett
January 1996,
Biology of the cell,
M P Sparrow, and
H W Mitchell, and
A W Everett
November 1989,
The Journal of biological chemistry,
M P Sparrow, and
H W Mitchell, and
A W Everett
August 1986,
Biochimica et biophysica acta,
M P Sparrow, and
H W Mitchell, and
A W Everett
January 1990,
Progress in clinical and biological research,
M P Sparrow, and
H W Mitchell, and
A W Everett
December 1987,
Circulation research,
