-transparent.png){kind=logo}
A simple and rapid colorimetric method for the assay of erythrocyte and plasma glutathione reductase (GR) activity is described. The method is based on the colorimetric measurement of reduced glutathione (GSH) (1) produced when the enzyme is incubated with oxidised glutathione (GSSG) in the presence of either reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH). Results of investigations on the effects of substrate and coenzyme concentrations, pH, EDTA, sodium/potassium chloride, and time, on enzyme activity are presented. Erythrocyte and plasma NADH-GR and NADPH-GR activities in 100 healthy blood donors, and 85 cord blood samples and plasma NADH-GR and NADPH-GR levels in patients with various disease conditions are given.
L Kum-Tatt, and
I K Tan, and
A M Seet
October 1968,
Analytical biochemistry,
L Kum-Tatt, and
I K Tan, and
A M Seet
August 1968,
The Journal of laboratory and clinical medicine,
L Kum-Tatt, and
I K Tan, and
A M Seet
December 2012,
Fish physiology and biochemistry,
L Kum-Tatt, and
I K Tan, and
A M Seet
April 1963,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
L Kum-Tatt, and
I K Tan, and
A M Seet
March 1975,
Zeitschrift fur klinische Chemie und klinische Biochemie,
L Kum-Tatt, and
I K Tan, and
A M Seet
August 1977,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
L Kum-Tatt, and
I K Tan, and
A M Seet
March 1965,
The Biochemical journal,
L Kum-Tatt, and
I K Tan, and
A M Seet
June 1963,
Wiener medizinische Wochenschrift (1946),
L Kum-Tatt, and
I K Tan, and
A M Seet
July 1960,
The Journal of laboratory and clinical medicine,
L Kum-Tatt, and
I K Tan, and
A M Seet
June 1998,
Protein expression and purification,
