-transparent.png){kind=logo}
Transient changes in the concentration of intracellular free calcium are associated with the transduction of primary signals and the subsequent employment of Ca2+ as a second messenger in a multitude of cell types. These transients, typically monitored with the calcium-sensitive fluorescent dye Fura-2, are known to occur with a time course in the order of seconds. In order to accurately monitor such rapid changes in intracellular free calcium concentration in both single cells and simultaneously in several cells in a single field, we have developed a digital fluorescence imaging system based on a charge-coupled device (CCD) camera. We report here on the detailed kinetics of calcium increases in cultured arterial swine smooth muscle cells in response to the agonist ATP.
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
March 1987,
Biochemical and biophysical research communications,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
August 1985,
Science (New York, N.Y.),
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
October 1986,
Biochemical and biophysical research communications,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
January 1988,
Annals of the New York Academy of Sciences,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
May 1997,
The American journal of physiology,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
August 1995,
The American journal of physiology,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
May 1986,
Biochemical and biophysical research communications,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
March 1991,
Circulation research,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
January 1996,
Clinical and experimental pharmacology & physiology,
J J Linderman, and
L J Harris, and
L L Slakey, and
D J Gross
November 1992,
The American journal of physiology,
