BIOMAT Database Logo BIOMAT Database Logo

Identification and characterization of an adenovirus 2 major late promoter CAP sequence DNA-binding protein. 1990

S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Laboratory of Molecular Hematology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

DNase I footprint analysis of the core adenovirus 2 (Ad2) major late promoter (MLP) has revealed distinct patterns of protection corresponding to the assembly of transcription components during transcriptional initiation (VanDyke, M. W., Sawadogo, M., and Roeder, R. G. (1989) Mol. Cell Biol. 7, 3371-3379). By using partially purified transcription factors, DNase I protection over the TATA box element and the CAP sequence was attributed to the binding of a single factor, TFIID. We have determined, however, that protection of the CAP region results from the binding of a novel factor, designated CAP-site binding factor (CBF), which is chromatographically and functionally distinct from TFIID. DNase I footprint analysis and gel electrophoresis mobility shift competition assays confirm that distinct polypeptides bind to the Ad2 MLP upstream promoter sequence, TATA box, and CAP sequences. When the CAP sequence is mutated, transcriptional activity of the Ad2 MLP is reduced both in vitro and in vivo. The decrease in transcriptional activity correlates with decreased CBF binding activity. Nuclear extracts depleted of CBF also exhibit reduced Ad2 MLP transcriptional activity. The addition of DNA affinity purified CBF, free of TFIID or major late transcription factor, restores the activity to control levels.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D003850 Deoxyribonuclease I An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA. DNase I,Streptodornase,DNA Endonuclease,DNA Nicking Enzyme,DNAase I,Dornavac,Endonuclease I,Nickase,Pancreatic DNase,T4-Endonuclease II,T7-Endonuclease I,Thymonuclease,DNase, Pancreatic,Endonuclease, DNA,T4 Endonuclease II,T7 Endonuclease I
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins

Related Publications

S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Identification of sequence-specific DNA-binding factors by label transfer: application to the adenovirus-2 major late promoter.
January 1988, Nucleic acids research,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Identification of a downstream sequence and binding protein that regulate adenovirus major late promoter transcription in vitro.
July 1988, The Journal of biological chemistry,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Interaction of CAP sequence site binding factor and transcription factor IID preceding and following binding to the adenovirus 2 major late promoter.
June 1991, The Journal of biological chemistry,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
The adenovirus type 2 DNA-binding protein interacts with the major late promoter attenuated RNA.
March 1989, Journal of virology,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
[Characteristics of the protein specifically binding to the major late promoter of adenovirus type 2].
January 1987, Doklady Akademii nauk SSSR,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.
February 1981, Proceedings of the National Academy of Sciences of the United States of America,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Sequence-specific methylation in a downstream region of the late E2A promoter of adenovirus type 2 DNA prevents protein binding.
November 1989, Journal of molecular biology,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Protein factor(s) binding independently to two different regions of the adenovirus 2 major late promoter.
May 1986, Nucleic acids research,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
Modification of an adenovirus major late promoter-binding factor during poliovirus infection.
September 1989, Journal of virology,
S Garfinkel, and J A Thompson, and W F Jacob, and R Cohen, and B Safer
The major late promoter and bipartite leader sequence of fowl adenovirus.
January 1998, Archives of virology,
Copied contents to your clipboard!