Biomaterial Database

Three-dimensional structure of intracellular membranous organelles in embryonic chick skeletal muscle cells in vitro revealed with scanning electron microscope. 1990

M Shiozaki, and Y Shimada

Department of Anatomy and Cell Biology, School of Medicine, Chiba University, Japan.

Using the freeze-polishing and osmium-maceration procedures, the ultrastructure of intracellular membranous organelles in embryonic chick skeletal muscle cells in vitro was investigated three-dimensionally with the scanning electron microscope (SEM). T-system tubules followed a tortuous course and possessed many warts. Some of these tubules could be traced to the sarcolemma. The sarcoplasmic reticulum (SR) had elaborate structures of a hexagonal pattern. By comparing the SEM images with those of ultrathin sections of myotubes impregnated with ferritin particles, the distinction between the T-system and SR was confirmed. Late in the culture, these two intracellular membranous systems were arranged periodically at right angles to the longitudinal axis of the myotubes. Round-shaped mitochondria were often arranged in chains connected with slender rods. Long rod-shaped mitochondria exhibited occasional branchings.

UI	MeSH Term	Description	Entries
D007425	Intracellular Membranes	Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.	Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015388	Organelles	Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.	Organelle