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Monoclonal antibody against membrane protein of transmissible gastroenteritis virus. 2013
Xuejiao Sun, and
Yudong Ren, and
Yu Li, and
Jiayi Zhu, and
Weijuan Zhu, and
Fan Ding, and
Guangxing Li, and
Chunfeng Wang, and
Ming Gao, and
Yunhang Gao, and
Liyan Cao, and
Xiaofeng Ren
College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China.
Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus that can cause piglet diarrhea with high mortality rates. TGEV membrane (M) protein not only plays a vital role in the process of virus assembly and budding, but also induces the production of interferon-α during infection. In this study, a monoclonal antibody (MAb) designated 7G7, against the TGEV M protein was generated by inoculating BALB/c mice with TGEV followed by hybridoma technique. Immunofluorescence assays indicated that MAb 7G7 was capable of detecting cell infection by TGEV. Virus-based ELISA demonstrated that MAb 7G7 can be used as a highly specific diagnostic reagent for TGEV.
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