Ex vivo characterization of particle transport in mucus secretions coating freshly excised mucosal tissues. 2013

Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.

Sustained drug delivery to mucosal surfaces has the potential to improve the effectiveness of prophylactic and therapeutic treatments for numerous diseases and conditions, including inflammatory bowel disease, sexually transmitted diseases, cystic fibrosis, glaucoma, dry eye, and various cancers. Sustained delivery systems such as nanoparticles can be useful for mucosal delivery, but recent work suggests they must penetrate the rapidly cleared mucus barrier that overlies all mucosal epithelia to achieve uniform distribution on epithelial surfaces and enhanced residence time. Thus, it is important to evaluate the mucus-penetrating ability of nanosized delivery systems in preclinical animal studies, and for administration to humans. We describe a simple ex vivo method to visualize and quantify nanoparticle transport in mucus on fresh mucosal tissues. Using this method in murine models, we observed variations in the mucus mesh at different anatomical locations, as well as cyclical variations that may have implications for mucosal delivery.

UI MeSH Term Description Entries
D007421 Intestine, Small The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM. Small Intestine,Intestines, Small,Small Intestines
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D009092 Mucous Membrane An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa. Lamina Propria,Mucosa,Mucosal Tissue,Muscularis Mucosae,Mucous Membranes,Membrane, Mucous,Membranes, Mucous,Mucosae, Muscularis,Mucosal Tissues,Propria, Lamina,Tissue, Mucosal,Tissues, Mucosal
D012137 Respiratory System The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about. Respiratory Tract,Respiratory Systems,Respiratory Tracts,System, Respiratory,Tract, Respiratory
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014132 Trachea The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi. Tracheas
D014621 Vagina The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed) Vaginas
D016503 Drug Delivery Systems Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity. Drug Targeting,Delivery System, Drug,Delivery Systems, Drug,Drug Delivery System,Drug Targetings,System, Drug Delivery,Systems, Drug Delivery,Targeting, Drug,Targetings, Drug
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
November 2015, Colloids and surfaces. B, Biointerfaces,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
May 2022, The Journal of investigative dermatology,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
July 2011, Physics in medicine and biology,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
August 2014, Biomedical optics express,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
April 2019, Journal of medical imaging (Bellingham, Wash.),
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
February 1980, IEEE transactions on bio-medical engineering,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
December 2021, Applied physics letters,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
October 2016, BMC medical imaging,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
December 2022, The Journal of the Acoustical Society of America,
Laura M Ensign, and Andreas Henning, and Craig S Schneider, and Katharina Maisel, and Ying-Ying Wang, and Marc D Porosoff, and Richard Cone, and Justin Hanes
January 2009, American journal of rhinology & allergy,
Copied contents to your clipboard!