A comparison of host-defense peptides in skin secretions of female Xenopus laevis × Xenopus borealis and X. borealis × X. laevis F1 hybrids. 2013

Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
Department of Biochemistry, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.

Peptidomic analysis was used to compare the diversity of host-defense peptides in norepinephrine-stimulated skin secretions from laboratory-generated female F1 hybrids of Xenopus laevis and Xenopus borealis (Pipidae). Skin secretions of hybrids with maternal X. laevis (XLB) contained 12 antimicrobial peptides (AMPs), comprising 8 from X. laevis and 4 from X. borealis. Magainin-B1, XPF-B1, PGLa-B1 CPF-B2, CPF-B3 and CPF-B4 from X. borealis and XPF-1, XPF-2, and CPF-6 from X. laevis were not detected and CPF-1 and CPF-7 were present in low concentration. The secretions contained caerulein and caerulein-B1 derived from both parents but lacked X. laevis xenopsin and X. borealis caerulein-B2. Skin secretions of hybrids with maternal X. borealis (XBL) contained 14 AMPs comprising 6 from X. borealis and 8 from X. laevis. Magainin-B1, XPF-B1, PGLa-B1, CPF-B2, XPF-1, CPF-5, and CPF-7 were absent and CPF-B3, CPF-B4, CPF-1 and CPF-6 were present only in low concentration. Xenopsin and caerulein were identified in the secretions but caerulein-B2 was absent and caerulein-B1 was present in low concentration. No peptides were identified in secretions of either XLB or XBL hybrids that were not present in the parental species. The data indicate that hybridization between X. laevis and X. borealis results in increased diversity of host-defense peptides in skin secretions but point to extensive AMP gene silencing compared with previously studied female X. laevis×X. muelleri F1 hybrids and no novel peptide expression.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009638 Norepinephrine Precursor of epinephrine that is secreted by the ADRENAL MEDULLA and is a widespread central and autonomic neurotransmitter. Norepinephrine is the principal transmitter of most postganglionic sympathetic fibers, and of the diffuse projection system in the brain that arises from the LOCUS CERULEUS. It is also found in plants and is used pharmacologically as a sympathomimetic. Levarterenol,Levonorepinephrine,Noradrenaline,Arterenol,Levonor,Levophed,Levophed Bitartrate,Noradrenaline Bitartrate,Noradrénaline tartrate renaudin,Norepinephrin d-Tartrate (1:1),Norepinephrine Bitartrate,Norepinephrine Hydrochloride,Norepinephrine Hydrochloride, (+)-Isomer,Norepinephrine Hydrochloride, (+,-)-Isomer,Norepinephrine d-Tartrate (1:1),Norepinephrine l-Tartrate (1:1),Norepinephrine l-Tartrate (1:1), (+,-)-Isomer,Norepinephrine l-Tartrate (1:1), Monohydrate,Norepinephrine l-Tartrate (1:1), Monohydrate, (+)-Isomer,Norepinephrine l-Tartrate (1:2),Norepinephrine l-Tartrate, (+)-Isomer,Norepinephrine, (+)-Isomer,Norepinephrine, (+,-)-Isomer
D002678 Chimera An individual that contains cell populations derived from different zygotes. Hybrids,Chimeras,Hybrid
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003433 Crosses, Genetic Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species. Cross, Genetic,Genetic Cross,Genetic Crosses
D005260 Female Females
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012867 Skin The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D014981 Xenopus An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.

Related Publications

Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
January 1979, Developmental biology,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
January 2012, Peptides,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
September 2014, Comparative biochemistry and physiology. Part D, Genomics & proteomics,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
January 1985, Peptides,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
October 2015, Peptides,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
August 2002, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
January 1986, Journal of natural products,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
November 2010, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
May 2012, General and comparative endocrinology,
Milena Mechkarska, and Manju Prajeep, and Jérôme Leprince, and Hubert Vaudry, and Mohammed A Meetani, and Ben J Evans, and J Michael Conlon
July 2011, Cellular and molecular life sciences : CMLS,
Copied contents to your clipboard!