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Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains. 2013
Jong Yul Roh, and
Jong Bin Park, and
Qin Liu, and
Song Eun Kim, and
Xueying Tao, and
Tae Woong Choi, and
Jae Young Choi, and
Woo Jin Kim, and
Byung Rae Jin, and
Yeon Ho Je
Department of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea. rohling@korea.kr
We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.
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