1. Glycogen phosphorylase-alpha, a commonly used index of cytosolic free calcium, was compared in various preparations of rat liver in the absence and presence of 0.1 microM epinephrine. 2. Total phosphorylase in isolated perfused livers and freshly-isolated hepatocytes were the same as that observed in liver in situ; however, phosphorylase-alpha was 50% higher in perfused liver and 80% higher in hepatocytes than activities measured in situ. Total phosphorylase was reduced approximately 50% in hepatocytes maintained in primary culture for 24 hr. 3. Epinephrine increased phosphorylase-alpha approximately 2-fold in livers perfused for 30 min but only about 20% in hepatocytes incubated for 30 min. After 90 min of perfusion or incubation, epinephrine increased phosphorylase-alpha nearly 4-fold in perfused livers and only 30% in isolated hepatocytes. The results suggest that amounts of free calcium and calcium-dependent coupling of adrenergic receptors to phosphorylase-alpha differ markedly between the intact liver and isolated hepatocytes.