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[Specificity of the degradation and synthesis of dinucleoside monophosphates by RNAase C2 of Asp. clavatus]. 1975

S I Bezborodova, and V I Guliaeva, and V G Morozova

The ability of RNAase E2 to degradate dinucleoside moniphosphates and to form internucleotide bonds was studied. The compounds of the GpN type were found to be a good substrate for RNase C2. The pH optimum for GpC was 5.5 (acetate buffer) and the temperature optimum was 30 degrees C. The values of Km and Vmax on GpC, GpA, GpG and GpU were determined. The affinity of the substrates for the enzyme decreased in the sequence GpC greater than GpG greater than GpA GREATER THAN GpU. RNase C2 catalyze the synthesis of GpC and GpU. The yield of GpC amounted to 60% and that of GpU was 35%. These data indicate that RNase C2 FROM Asp. clavatum is guanyl ribonuclease (EC 3.1.4.8.).

UI MeSH Term Description Entries
D009841 Oligonucleotides Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed) Oligonucleotide
D006163 Ribonuclease T1 An enzyme catalyzing the endonucleolytic cleavage of RNA at the 3'-position of a guanylate residue. EC 3.1.27.3. Guanyloribonuclease,RNase T1,Ribonuclease N1,Aspergillus oryzae Ribonuclease,Guanyl-Specific RNase,RNase Apl,RNase F1,RNase Pch 1,RNase ST,Ribonuclease F1,Ribonuclease F2,Ribonuclease ST,Ribonuclease T-1,T 1 RNase,Guanyl Specific RNase,RNase, Guanyl-Specific,RNase, T 1,Ribonuclease T 1,Ribonuclease, Aspergillus oryzae
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001230 Aspergillus A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
D012260 Ribonucleases Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-. Nucleases, RNA,RNase,Acid Ribonuclease,Alkaline Ribonuclease,Ribonuclease,RNA Nucleases,Ribonuclease, Acid,Ribonuclease, Alkaline
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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